基于MAPK信号通路探讨硒代蛋氨酸缓解呕吐毒素致仔猪肠道损伤的分子机制

中国是全球最大的生猪养殖国，而仔猪生产是生猪养殖中的核心环节。猪是对呕吐毒素最敏感的动物，饲料呕吐毒素污染严重威胁仔猪的生长和健康，给生猪养殖业带来巨大的经济损失。近年来，有关硒缓解呕吐毒素致畜禽毒性作用中的研究成为热点，但硒代蛋氨酸在缓解呕吐毒素致仔猪肠道损伤中的作用及最佳饲粮添加水平未见报道，且硒代蛋氨酸缓解呕吐毒素致仔猪肠道损伤的作用机制尚不清楚。因此，本项目拟从机体（仔猪）、组织（仔猪空肠离体组织）、细胞（IPEC-J2细胞）和分子水平（信号分子MAPK）系统深入地探讨硒代蛋氨酸缓解呕吐毒素致仔猪肠道损伤的分子机制，以期（1）明确硒代蛋氨酸在缓解呕吐毒素致仔猪肠道损伤中的作用，并确定硒代蛋氨酸在缓解呕吐毒素致仔猪肠道损伤的最佳饲粮添加水平；（2）阐明硒代蛋氨酸缓解呕吐毒素致仔猪肠道损伤的分子机制。研究结果将为生猪养殖中缓解呕吐毒素致仔猪毒性作用提供理论依据和实践参考。

China is the largest pork-producing country in the world, and piglet production is the key phase in the swine industry. Pig is the animal most sensitive to deoxynivalenol, and deoxynivalenol contamination in feed seriously threatens the growth and health of piglets, which leads to huge economic losses in the swine industry. In recent years, the research of selenium in alleviating the toxicity of mycotoxins in livestock has become a hot spot. However, little is known about the effects of selenomethionine in alleviating the intestinal damage caused by deoxynivalenol and the optimal dietary amount of selenomethionine against the toxicity, as well as the underlying action mechanism. Therefore, this project intends to systematically explore the molecular mechanism of selenomethionine in alleviating the intestinal damage induced by deoxynivalenol at the animal (piglet), explant tissue (jejunal explant), cell (IPEC-J2 cells), and molecule (MAPK signaling molecules) levels. This project aimed to (1) clarify the role of selenomethionine in alleviating the intestinal injury caused by deoxynivalenol in piglets, and determine the optimal dietary selenomethionine amount against the deoxynivalenol toxicity; (2) clarify the molecular mechanism of selenomethionine in alleviating the intestinal injury of piglets caused by deoxynivalenol. The project will provide a theoretical basis and practical reference for alleviating the toxic effects of deoxynivalenol on piglets in the swine industry.