circRNA-VDAC参与心肌缺血再灌注损伤的调控作用及其机制

心肌缺血再灌注损伤(MIRI)是心脏体外循环术后常见的损害，其发生是一个涉及多因素的复杂过程，需要更全面、更深入的科学研究。CircRNA通过对miRNA的调控，在心血管疾病的病理生理中发挥着重大作用，但其在心肌缺血再灌注中的作用尚未报道。我们在前期研究中通过体外和体内模型发现circRNA-VDAC在心肌缺血再灌注过程中表达显著上调，初步的功能研究也提示在细胞中抑制circRNA-VDAC的表达能改善缺血再灌注导致的细胞损伤。上述结果提示circRNA-VDAC参与缺血再灌注损伤过程，但是其功能和具体机制尚不明确。在此基础上我们将从circRNA-miRNA、circRNA-蛋白、circRNA-DNA等不同角度进一步深入研究circRNA-VDAC参与心肌缺血再灌注损伤的机制，初步探索circRNA-VDAC作为调控靶点的前景以及临床检测标志物的可能性。

Myocardial ischemia / reperfusion injury (MIRI) is a common cardiac injury after cardiopulmonary bypass (CPB). The occurrence of myocardial ischemia reperfusion injury is a complex process involving many factors, which requires more comprehensive and in-depth scientific research. Studies have shown that circRNA is involved in the process of different cardiac diseases by controlling miRNA, but whether it has played a role in myocardial ischemia and reperfusion has not been reported. We found that circRNA-VDAC expression was significantly up-regulated during myocardial ischemia and reperfusion in previous studies in vitro and in vivo model, cell damage function preliminary study also suggested that inhibition of circRNA-VDAC expression in the cell can improve ischemia reperfusion induced. These results suggest that circRNA-VDAC is involved in the process of ischemia reperfusion injury, but its function and mechanism are not clear. Through this study, we will clarify the mechanism of circRNA-VDAC in myocardial ischemia reperfusion injury through studying the function of circRNA-miRNA，circRNA-protein and circRNA-DNA , and the possibility of circRNA-VDAC as a mark of clinical treatment and detection.