纳米氧化钼局域表面等离子共振效应的可逆调控及生物分析应用研究

本项目针对基于纳米贵金属的比色生物分析，在准确性、重现性、线性范围、稳定性、成本、材料重复利用方面的不足，拟制备不同尺寸、形貌、维度及厚度的氧化钼纳米材料；借助酶反应原位产生还原剂与其反应，引入氧空位或氢原子对其掺杂，产生局域表面等离子效应；通过氧化剂处理，补偿氧空位或氢原子被氧化而去掺杂，实现局域表面等离子共振吸收的可逆调控；通过理论和实验研究，揭示局域表面等离子效应形成与调控机制；将其与生物识别反应偶联，通过酶促反应、酶标记抗体或DNA，用于酶法分析、酶联免疫分析和核酸分析，并采用适当的放大技术如核酸杂交连锁反应放大、多酶级联放大等，发展高灵敏、高选择性、低成本的比色生物分析方法，用于检测糖尿病、高尿酸血症、急性心肌梗死、癌症等的生物标志物。通过本项目的研究，将有望克服传统基于纳米贵金属比色生物分析的不足，为相关疾病早期发现、诊断、治疗、预后评估和疗效检测提供技术支撑。

In order to overcome the drawbacks of the reported colorimetric bioassays based on the noble metal nanomaterials in terms of reproducibility, accuracy, linear range, stability, cost, and reuse, in this project, the molybdenum oxide (MoO3) nanomaterials with different size, morphology, dimension, and thickness will be prepared. And the localized surface plasmon resonance (LSPR) of MoO3 nanomaterials will be generated by doping of oxygen vacancy and hydrogen atom from biological reducing agents. Then, the doped MoO3 nanomaterials will be oxidized for dedoping, realizing a reversible tunability of the LSPR in MoO3 nanomaterials. The mechanism of the formation and tunable LSPR will be studied by theoretical and experimental methods. Moreover, the MoO3 nanomaterials will be coupled with enzymatic reactions, enzyme-linked immunosorbent assay, and DNA assay under the amplification techniques to develop colorimetric methods for highly sensitive, selective, low-cost detection of the biomarkers of various diseases such as diabetes, hyperuricemia, acute myocardial infarction, and cancers. According to the study of this project, the shortcoming of the reported colorimetric bioassays may be eliminated, which may be provide an important technology support for the early detection, diagnostic, therapy, and prognostic evaluation of the related diseases.