采后哈密瓜对曲霉病原菌侵染的分子应答机制研究

哈密瓜是新疆特色瓜果，贮运过程中极易受到病原菌的侵染，采后哈密瓜应对病原菌侵染的反应机制的理解和调控是提高其贮藏品质和延长贮藏时间的关键。本项目拟采用蛋白质组学、转录组学及现代分子生物信息学等技术手段，研究采后哈密瓜对曲霉病原菌侵染的应答反应，深入考察曲霉病原菌侵染前后哈密瓜微观结构、生理生化、果实总蛋白质组、基因转录组水平的差异变化；初步鉴定与病程相关的重要差异蛋白质，分析其主要功能作用；揭示曲霉侵染过程中哈密瓜差异表达基因种类、功能和数量，明确差异蛋白、差异表达基因与曲霉侵染过程的关联性。项目研究结果将有助于发现并鉴定与哈密瓜抗病性相关的重要差异蛋白质和基因；从mRNA和蛋白质的表达水平阐明采后哈密瓜对曲霉病原菌侵染的应答机制，为开展相关抗病性基因的表达调控奠定基础，进而为哈密瓜采后病害的无污染防治技术和绿色保鲜技术，提供理论依据。

Hami melon is characteristic amphisarca in Xinjiang, postharvest melon is highly infected by pathogen during storage and transportation.Understanding and regulation of mechanism of postharvest Hami melon response to the pathogenic microorganism infection is the key for improving storage quality and prolonging the storage time. The project prepares to study postharvest Hami melon response to the pathogenic microorganism infection using proteomics, transcriptomics, bioinformatics technology, etc. The variation of microscopic structure, physiologic and biochemical index, proteome and differential gene expression on transcription level in Hami melon are investigated thoroughly, before and afte they are infected by the pathogenic microorganism.The major differential pathogenesis-related protein is identified preliminarily, then its main function and quantity are analysed. We will reveal the type, function and quantity of the different expression gene, and verify the relevance among differential protein, different expression gene and infection process.The results will help us to find and identify the important pathogenesis-related differential protein and gene, clarify the mechanism of postharvest Hami melon response to Aspergillus pathogenic microorganism infection on the mRNA and protein expression level,and establish a foundation for expression and regulation of disease resistance related gene, then provide the theory basis for no pollution control and green fresh-keeping technology for postharvest Hami melon.