心复康口服液对心衰大鼠心肌线粒体内膜功能及能量代谢蛋白质组学研究

心力衰竭是临床循环系统疾病发生发展到终末期出现的严重并发症。其中心肌结构重构是心衰难以逆转的病理基础，而能量代谢重塑是先于结构重塑的病理表现。线粒体在各种因素造成的心肌细胞缺血缺氧过程中又是最先出现功能变化及损害的细胞器。研究已证实，心复康口服液对线粒体功能结构、能量代谢、能量物质的合成、能量物质的转运等环节均有不同程度的阳性干预作用，有很好的逆转心力衰竭是心肌细胞线粒体代谢障碍，能量物质生成障碍，能量物质转运障碍的作用，拮抗心力衰竭是心肌结构及功能重构作用显著。本研究以线粒体能量合成及转运的关键部位-线粒体内膜为研究对象，对内膜运用高通量的研究方法--ITRAQ蛋白组学技术，就心复康口服液对内膜氧化磷酸化及能量物质转运过程中各种酶及蛋白质的进行研究，并对线粒体功能进行测定，真实还原线粒体能量代谢障碍的蛋白质表达。对心复康治疗心力衰竭心肌线粒体能量代谢相关蛋白质机制给予研究。

Heart failure is a clinical diseases of the circulatory system to develop into a serious complication of end-stage. Myocardial structural remodeling of heart failure is unlikely to reverse the pathological basis, energy metabolism remodeling before remodeling pathological manifestations. Mitochondria as organelles only energy synthesis plant, the process of myocardial ischemia and hypoxia caused by a variety of factors, the first thing that the functional changes and damage cells. Studies have confirmed positive intervention effect on mitochondrial function structure, energy metabolism, the synthesis of the energy substances, energy substances transporters and other sectors have different levels of, Xinfukang oral solution, the reversal of heart failure is good myocardial mitochondrial metabolic disorders energy substances generated obstacles, the role of energy material transport barriers antagonistic heart failure is significant myocardial structure and function of remodeling. Mitochondrial energy production and transit of critical parts of the study - study mitochondrial inner membrane of the intima use of high-throughput research methods - ITRAQ proteomics technology, Kang oral liquid on heart complex endometrial oxidative phosphorylation and transport processes of energy substances without missing a variety of enzymes to conduct a comprehensive study of protein expression in the real reduction of mitochondrial energy metabolism. Give a comprehensive study of the Heart Rehabilitation treatment of heart failure myocardial mitochondrial energy metabolism.