新资源食品广东虫草C6-ZFP转录因子CgPRO1功能研究

广东虫草是申请人所在团队发现的我国特有虫草新资源，已获国家卫生部批准为新资源食品，具有多种显著的活性功效，在食品、保健品及医药等领域有广阔的应用前景。因此对其子实体发育及活性成分调节进行深入研究非常必要。C6-ZFP转录因子PRO1在调节真菌子实体发育及代谢过程中具有重要作用，且其调节的下游靶蛋白参与多个发育及代谢相关信号途径，但虫草真菌中该转录因子具体功能未见报道。为探究广东虫草子实体发育及活性成分调节机制，本项目将开展广东虫草C6-ZFP转录因子PRO1（CgPRO1）功能研究，明确该基因在其子实体发育不同阶段的表达规律，并通过分子遗传学方法对该基因进行缺失突变及过量表达，通过与野生型菌株子实体发育形态比较，明确该基因在广东虫草子实体发育过程中的调节作用，并通过代谢组分析深入探究该基因是否参与其代谢调节以及参与的具体代谢途径，为深入解析广东虫草子实体发育及活性成分调节机理提供基础。

Cordyceps guangdongensis is a unique Cordyceps fungus discovered by our research team, which has been approved as a Novel Food by the Chinese Ministry of Public Health. It has a broad application prospect in food, health products and pharmaceutical industries due to its rich nutrients content and significant pharmacological activities. Therefore，it is urgent necessary for conduct further research on the regulating mechanism of fruiting body development and metabolic processes of bioactive compounds. Previous studies have shown that Zn2Cys6-type (C6-ZFP) transcription factor PRO1 and its homologous protein were involved in fruiting body development in many groups of macrofungi. And its regulated downstream target proteins are involved in multiple developmental and metabolic signaling pathways. However, the function of C6-ZFP transcription factor PRO1 in Cordyceps remains unknown. In order to gain new insights into fruiting body development and metabolic processes of bioactive compounds, this project will be focused on the function of C6-ZFP transcription factor PRO1 of C. guangdongensis. The expression pattern of C6-ZFP transcription factor PRO1 during different stages of fruiting body development will be analyzed, and mutants of PRO1 deletion and overexpression strains will be generated to further analyze the function of PRO1 by comparison the morphology of fruiting body formation to the wild type. Furthermore, metabolomics analysis will be used to explore whether the C6-ZFP transcription factor PRO1 is involved in metabolic processes of bioactive compounds and detailed metabolic pathways. The prospective results will be basic foundation for effective exploration of regulating mechanism of fruiting body development and metabolic processes of bioactive compounds in C. guangdongensis.