circRNA_0012891通过吸附miR-17-5p/miR-106a-5p释放MMP2促进恶性黑色素瘤转移的分子机制研究

转移是导致恶性黑色素瘤患者死亡的首要原因但其发生机制尚不完全清楚。我们通过芯片发现circRNA_0012891在转移性黑色素瘤组织中高表达，过表达circRNA_0012891提高了恶性黑色素瘤的侵袭能力。生物信息学预测circRNA_0012891可以吸附多种microRNAs释放相关靶分子发挥功能，根据前期基础我们提出 “circRNA_0012891通过吸附miR-17-5p/miR-106a-5p释放MMP2促进恶性黑色素瘤转移”的假说，拟从临床样本、细胞及动物水平上开展研究，交叉使用分子生物学（IHC、qRT-PCR、WB、荧光素酶实验、RIP及RNA pull down等）、生物信息学及分子影像学（PET-CT）等手段验证上述假说。本项目将初步揭示circRNA_0012891促进恶性黑色素瘤转移的分子机制，也为发掘新的诊断及治疗靶点、开发新的分子影像探针奠定理论基础。

Metastasis is the leading cause of death in patients with malignant melanoma, but the mechanism is not fully understood. We found that circRNA_0012891 is highly expressed in metastatic melanoma tissue by the chip, and overexpression of circRNA_0012891 improves the invasive ability of malignant melanoma. Bioinformatics predict that circRNA_0012891 can adsorb a variety of microRNAs to release related target molecules to function. According to the previous basis, we propose the hypothesis that circRNA_0012891 promotes malignant melanoma metastasis by adsorbing miR-17-5p/miR-106a-5p to release MMP2. We will conduct research at clinical, cell and animal levels, cross-use molecular biology (IHC, qRT-PCR, WB, luciferase assay, RIP and RNA pull down, etc.), bioinformatics and molecular imaging (PET-CT) and other means to verify the above hypothesis. This project will initially reveal the molecular mechanisms by which circRNA_0012891 promote the metastasis of malignant melanoma, and lay a theoretical foundation for the discovery of new diagnostic and therapeutic targets and the development of new molecular imaging probes.