EHV-1囊膜蛋白gD高尔基体驻留功能的分子机制研究

马疱疹病毒1型(EHV-1)的囊膜形成机制还不清楚，阻碍了相关疾病的防治及新型疫苗的研发。EHV-1与其它疱疹病毒(如HSV-1和PRV等)不同，其囊膜蛋白gD表达后会驻留于高尔基体内并直接参与囊膜的形成，然而其机制目前尚未见报道。我们的前期研究结果显示，gD蛋白的FD(Functional domain)及CT(Cytoplasmic terminal)两个功能区在定位功能上起关键作用。因此，本项目将继续从gD高尔基体驻留信号序列的鉴定、gD蛋白胞质区磷酸化位点及宿主细胞靶蛋白三个方面，研究gD高尔基体驻留的分子机制。本研究将为阐明EHV-1囊膜形成机制及新型VLP嵌合疫苗的研发奠定理论基础。

It remains unclear about the mechanism of envelopment of Equine herpesvirus type 1 (EHV-1), which not only hinders the prevention and treatment of relevant diseases but also blocks the development of new vaccines. The envelope protein gD encoded by EHV-1, whose localization is different from that of other herpesvirus (e.g. HSV-1 and PRV), will be detained in Golgi network and participated directly in the envelopment after expression, however, the mechanism has not been reported so far. Our previous studies revealed that the FD and CT domain play a key role in the localization of gD. We will continue focusing on three aspects including identification of gD Golgi-retention signal (GRS), effects of phosphorylation sites in CT domain and host cell target protein(s) on gD Golgi retention function, in order to study the molecular mechanism of the Golgi-retention function. Our studies will provide theoretical basis for elucidating the mechanism of envelopment and develop new chimeric VLP vaccines.