基于lncRNA-miRNA-mRNA研究线粒体Bcl-2基因在骨质疏松发病中的作用及中药对其影响

骨质疏松是一种随年龄增长，逐渐发生的慢性衰老性骨代谢疾病。线粒体是细胞生命活动和细胞凋亡的控制中心。Bcl-2基因在调控线粒体功能中有重要作用，但其调控线粒体凋亡的机制目前仍未完全阐明，其在骨质疏松发病中的作用研究更少。本课题在前期研究基础上，从lncRNA-miRNA-mRNA着眼，（1）探讨骨质疏松发生时microRNA/lncRNA array表达差异情况，针对Bcl-2进行ceRNA技术分析，构建骨质疏松相关的ceRNA（lncRNA-miRNA-mRNA）网络图，并对目标lncRNA、miRNA验证。（2）将目标miRNA类似物（mimic）和针对目标lncRNA的小干扰RNA转染人成骨细胞，观察目标lncRNA、目标miRNA、Bcl-2 mRNA 的变化。（3）借助上述转染后人成骨细胞模型，研究补肾健脾活血方对目标lncRNA、目标miRNA和Bcl-2mRNA的影响。

Osteoporosis is a chronic senescet metabolic bone diseases along with the age growth.Mitochondria is the control center of cell about life activity and apoptosis.Bcl-2 gene plays a significant role in the regulation of mitochondrial function, however the regulation of mitochondrial apoptosis mechanism is still not yet fully understood, especially the role of causing osteoporosis is seldom researched.This topic focuses on lncRNA-miRNA-mRNA based on the previous research,(1)To observe the expression differences of microRNA/lncRNA array while osteoporosis occurs, the network diagram of osteoporosis related ceRNA (lncRNA-miRNA-mRNA) is structured by ceRNA technology analysis in view of the Bcl-2, then the target lncRNA and miRNA will be checked.(2)Osteoblast is transfected by the target of micrornas analogues (mimic) and the small interference RNA of the target lncRNA ,then the change of the target lncRNA, miRNA and Bcl-2 mRNA will be observed.(3)To explore the impact Bushenjianpihuoxue prescribe on the target lncRNA,the target miRNA and Bcl-2 mRNA through above the mode cell of the transfection posterity osteoblast.