基于非线性理论的房颤痰火扰心证自主神经调控起搏基因表达的研究

前期研究中我们发现，房颤痰火扰心证与自主神经功能关系密切。本研究以非线性理论为基础，从交感－迷走学说出发，提出假说：房颤时，大鼠左房胆碱能神经和肾上腺素神经分布密度变化明显，自主神经调控下游的超极化激活的环苷酸门控阳离子通道基因HCN2、HCN4在心脏不同部位（窦房结、心房、心室）表达的差异性是房颤的原因之一。山东省中医院院内制剂青山健心片治疗房颤的作用靶点在于抑制交感神经、影响下游起搏基因表达。围绕假说，利用poincare数模，将多种层次（器官、分子、超微结构）和多种手段（数模分析、心电效应、蛋白质组学、基因工程方法）相结合的方法，从功能和形态学相结合的方面探讨了房颤痰火扰心证与自主神经的关系。利用基因工程方法，将HCN4转染大鼠骨髓间充质干细胞，提高了实验的稳定性和可重复性，为中药治疗房颤提供了新思路。

In previous research, we found that the relationship between atrial fibrillation phlegmsyndrome and autonomic nervous function closely. Based on nonlinear theory, this study proposes a hypothesis from the perspective of sympathetic vagal Theory: at the time of atrial fibrillation, the distribution density of cholinergic nerves and adrenergic nerves in the left atrial，downstream of the autonomic nervous regulation of the hyperpolarization activated ring nucleotide gated cation channel gene HCN2 and HCN4 expression in different parts of the heart (sinus node, atrium, ventricle) differential expression is one of the causes of atrial fibrillation。The target of the treatment of atrial fibrillation with QingShanJianXin is to inhibit sympathetic nerve and affect the expression of the downstream pacemaker gene. Around the hypothesis that the use of digital to analog method, method of combining multiple (organ, molecular and ultrastructural) levels, and a variety of means (digital to analog analysis, ECG effect, proteomics, genetics method), from the combination of functional and morphological aspects to explore the relationship between atrial fibrillation of phlegm fire disturbing heart syndrome and autonomic nerve. Using genetic methods, HCN4 transfection of rat bone marrow mesenchymal stem cells, improve the stability and repeatability, provides a new way for traditional Chinese medicine in the treatment of atrial fibrillation.