源于云南农村白族汉氏杆菌在溃疡性结肠炎中的作用及机制研究

肠道菌群失调在溃疡性结肠炎（UC）发病机制中起重要作用，但关键菌群发挥的功能与宿主免疫间的互作却知之甚少。我们前期发现：①UC患者肠道中汉氏杆菌较健康人显著降低；②云南农村白族UC发病率低于汉族，汉氏杆菌丰度显著升高；③与城市汉族相比，农村白族为粪菌移植（FMT）供体治疗UC，疗效更好，移植后汉氏杆菌丰度显著升高并定植12周以上；④预实验发现汉氏杆菌可能通过调控Th17/Treg平衡减轻DSS诱导的小鼠肠炎。但汉氏杆菌调控Th17/Treg平衡在UC中的具体机制不明。故本项目拟通过：临床水平：扩大样本选择农村白族为FMT供体治疗UC，研究汉氏杆菌的定植水平和Th17/Treg细胞比例；动物水平：探讨其在DSS小鼠肠炎中的防治效应及对Th17/Treg平衡的影响；细胞水平：揭示其调控Th17/Treg平衡的具体机制。最终阐明汉氏杆菌在UC中的作用机理，为探索UC治疗的新靶点提供理论依据。

Gut microbiota dysbiosis plays an important role in the pathogenesis of ulcerative colitis (UC), but few people is known about the interaction between key gut microbiota functions and hosts immune. Preliminary findings: ①The intestinal phascolarctobacterium faecium(P. faecium) of UC patients was significantly lower than that of healthy patients. ②The incidence rate of the Bai ethnic group UC in Yunnan was lower than that of Han ethnic group, and the P. faecium abundance increased significantly. ③ Compared with the urban Han ethnic group, the rural Bai ethnic group was better treated UC which having FMT donor on it, and the P. faecium abundance increased significantly after FMT and was colonized for more than 12 weeks. ④ Pre-experiment showing that P. faecium may attenuate DSS induced enteritis in mice by regulating Th17/Treg balance. But the specific system of P. faecium regulation Th17/Treg balance in the UC is not clear. .1) Clinical studies: Expanding the sample to select the rural Bai ethnic group as the FMT donor treatment UC, studying P. faecium colonization level and Th17/Treg cell proportion;.2) Animal model:To investigate P. faecium control effect in DSS mice and its effect on Th17/Treg balance;.3) Cell culture studies: Revealing the effects and mechanisms of P. faecium regulation Th17/Treg balance..These studies are intended to investigate the mechanism of P. faecium in UC and provides a theoretical basis for exploring new targets of UC therapy.