大豆粒重基因GmSW1的克隆与功能分析

大豆（Glycine max）是我国重要的经济作物、粮食作物、饲料作物及工业原料，在国民经济中占有重要地位。本项目利用高产优质大豆品种齐黄34与冀豆17构建的重组自交系群体，通过图位克隆的策略，克隆控制大豆种子大小的关键基因GmSW1，解析大豆种子大小形成的分子机理。进一步利用栽培大豆种质资源，通过关联分析及功能验证，研究基因GmSW1驯化过程的作用及该基因所在育种选择区域在大豆品种选育过程中的实际效应。结合运用分子生物学和生物信息学技术，筛选和鉴定GmSW1的互作蛋白，解析GmSW1基因参与的大豆种子大小发育的遗传调控网络，为大豆粒形的精准分子设计育种，优良大豆品种的进一步遗传改良提供重要思路。

Soybean (Glycine max) is an economic crop, alimentary crop, feed crop and industrial raw material in China, which occupies an increasingly important position in the development of the national economy. This project is to isolate and characterize the gene GmSW1 which controls soybean seed weight by map-based cloning from a recombinant inbred line population developed by the cross between two high-yield and superior-quality soybean varieties Qihuang 34 and Jidou 17. And the molecular mechanism of seed size formation in soybean is elucidated. Meanwhile, cultivated soybean germplasm resources are used to analysis the function of gene GmSW1 and the actual effect of selection region of soybean breeding by association analysis and functional verification of target genes. And we screen and identify GmSW1 interaction protein with the confirmation of molecular biology and bioinformatics. The genetic regulatory network of seed size will be constructed successfully. In conclusion, this study would provide an important strategy for further genetic improvement of excellent soybean varieties and more accurate breeding by molecular design.