从lncRNA-PVT1调控细胞外基质成分研究益智仁-乌药干预糖尿病肾病的作用机制

DN作为终末期肾病元凶之一，其发病机制仍不明确，且治疗棘手。我们前期研究证实药对“益智仁-乌药”的石油醚提取部位在治疗DN中有较好的作用；研究表明lncRNA-PVT1 通过ECM的堆积而引起DN的发生和发展，据此提出益智仁-乌药通过干预lncRNA-PVT1，抑制ECM积聚，减轻肾小球病理改变和足细胞损伤这一假说。拟通过质粒构建、细胞转染及qPCR、Western blot和ELISIA等方法研究lncRNA-PVT1调控的ECM成分（Col-Ⅰ/Ⅳ，FN，LN）及相关信号通路（TGF-β1）和降解酶系（MMP-9、TIMP-1）的影响，从整体、组织、分子水平研究益智仁-乌药治疗DN 的可能机制，为运用中医药治疗DN 提供科学依据。

DN as one of the culprits of end-stage renal disease, its pathogenesis is still unclear, and the treatment of intractable. Our previous studies have shown that Petroleum ether fraction be the effective fractions of compatibility Yizhiren and Wuyao for anti-diabetic nephropathy. The results showed that long noncoding RNA pvt1 by ECM accumulation caused the occurrence and development of DN. Based on this, Yizhiren and Wuyao through the intervention of long noncoding RNA pvt1, inhibit ECM accumulation, reduce the pathological changes of glomerular podocyte injury this hypothesis. Quasi by plasmid, transfection and qPCR and Western blot and ELISIA etc. methods the long noncoding RNA pvt1 regulation of ECM components (Col I / IV, FN, LN) and the related signaling pathways (TGF-β1) and degradation enzyme system (MMP-9, TIMP-1), as a whole, organization, molecular level research study Yizhiren and Wuyao treating DN mechanism, provide a scientific basis for the application of the traditional Chinese medicine in the treatment of DN.