针刺抑制EGC网络的瀑布级联效应缓解IBS内脏高敏的机制研究

肠易激综合征（IBS）是发病率最高的一类功能性肠病。内脏高敏是IBS重要的病理机制之一。目前药物治疗症状反复，副作用明显，如何寻求安全有效的治疗方法是亟待解决的问题。针刺缓解IBS患者腹痛、腹胀等内脏高敏症状疗效肯定，但其作用机制有待深入研究。最新研究表明，肠道胶质细胞（EGC）活化诱发瀑布级联效应所致的EGC网络功能异常是促进和维持IBS内脏高敏发生发展的关键环节。本课题以IBS内脏高敏模型大鼠为研究对象，以促进和维持内脏高敏的EGC及其网络瀑布级联效应为切入点，从BDNF/TrkB-PLC-Ca2+信号通路入手，通过免疫组化、原位杂交结合计算机图像分析、RT-PCR等技术手段，围绕“针刺通过下调BDNF含量，抑制EGC活化和缝隙连接所形成的瀑布级联效应，改善EGC网络功能状态，缓解IBS内脏高敏状态”这一假说，深入探讨针刺治疗IBS内脏高敏的作用机制。

Irritable bowel syndrome (IBS) is one of most common functional bowel diseases. Visceral hypersensitivity is the most important pathological mechanism and manifestation of IBS. Nowadays, with the obvious side effects, find a safe and effective method is needed. It is reported that puncturing Tianshu with Taichong is able to relieve the symptom of abdominal pain and distention. However, the mechanism of acupuncture for IBS patients with visceral hypersensitivity is still unclear. Recent studies indicated that the dysfunction of EGC network resulting from cascade effect activated among triggered EGC is the key point in pathogenesis of visceral hypersensitivity. Studying on this network is probably a way to explore the visceral mechanism of peripheral. Therefore, we hypothesis that acupuncture can relieve the visceral hypersensitivity of IBS patients by regulate the EGC and cascade effect of its network. We plan to use IBS model rats with visceral hypersensitivity as the research subjects, focus on the point that acupuncture can reduce the level of BDNF, suppress the overactivity of EGC and its gup junctions which contribute to cascade effect, improve the function of EGC network, and relieve the visceral hyperactivity, utilize techniques such as immunohistochemistry, in situ hybridization with computer graphics analysis, RT-PCR to research on the regulation effects of acupuncture on visceral sensitivity.