黄麻耐盐性转录组及QTL分析研究

黄麻是世界上最重要的韧皮纤维作物之一，近年来，世界各国对黄麻的需求量不断增大。目前土壤盐渍化日趋严重，威胁着我国农业可持续发展。开展黄麻耐盐性研究，可为选育耐盐品种、扩大盐渍地黄麻种植奠定基础。前期研究中，我们已筛选获得2个耐盐性极端类型的黄麻种质：耐盐材料“O-3”和盐敏感材料“南阳长果”，并已获得“O-3”ד南阳长果”杂交F1代种子。本项目拟采用高通量测序技术，研究阐明在高中性盐浓度胁迫下耐盐性黄麻及盐敏感黄麻自身基因组表达变化情况及2个耐盐性极端类型种质间的基因组表达变化差异。并进一步利用基于转录组数据开发的分子标记，对190株“O-3”ד南阳长果”衍生F2群体进行基因分型，结合3年6环境苗期耐盐性相关表型数据进行耐盐性QTL分析。本项目的研究意义在于可以挖掘部分黄麻耐盐性基因，深入揭示其耐盐性分子机理，为黄麻的分子育种打下坚实的基础。

Jute (Corchorus) is one of the most important of bast fiber crops in the world. In recent years, there is an increasing demand of jute around the world. The trend of soil salinization is becoming much more serious, which is a serious threat to agricultural sustainable development in our country. It can lay the foundation for cultivating salt-tolerant varieties and planting on salt land to make more research in jute salt tolerance. In the previous studies, we have obtained 2 salt-resistance extreme types of jute germplasms: “O-3”, a salt-tolerace jute species, and “nanyangchangguo”, a salt-sensitive jute species, and seeds of F1 from “O-3”דnanyangchangguo”. In this study, we try to illustrate transcriptome difference between before and after neutral salt stress of the salt-tolerace jute and the salt-sensitive jute, and difference of transcriptome with difference between them by high-througout sequencing. And we will develop molecular markers using the transcriptome data. QTL analysis will be carried out using genotypes of 190 F2 plants derived from “O-3”דnanyangchangguo” and phenotypic datas related with salt tolerance of the plants in 3 years 6 environments in order to deeply analyze molecular mechanism of salt tolerance in jute and fully excavate excellent salt tolerance genes of jute, Which will lay a solid foundation for molecular breeding in jute.