天麻活性成分抗脑缺血再灌注损伤的线粒体机制

能量代谢障碍是脑缺血再灌注损伤（CIRI）复杂的病理环节中是最早、最直接、最迅速发生的病理改变，被认为是脑损伤的始动环节，主要表现在线粒体结构和功能的改变。我们前期发现天麻乙酸乙酯提取物（EEG）所含的3个活性成分能通过血脑屏障具有显著抗CIRI作用，并证实其作用机制涉及线粒体能量代谢障碍过程中的氧化损伤和细胞凋亡两个关键环节，提示天麻活成分在缺血性脑血管病(ICVD)发生和发展过程中可能通过调控线粒体途径而发挥抗CIRI作用。本课题拟以天麻3个入脑成分为研究对象，采用缺血再灌注损伤的动物及细胞模型，拟从改善线粒体功能障碍、维持线粒体融合分裂的动态平衡、激活线粒体自噬、促进新线粒体的形成、神经元/星形胶质细胞能量代谢的交互调控等方面入手，探讨天麻活性成分是如何调控线粒体相关因素构成的复杂能量代谢网络，阐释清楚天麻活性成分抗脑缺血再灌注损伤的线粒体机制。

The energy metabolism disorder is the earliest, most direct and rapid pathological changes on cerebral ischemia reperfusion injury（CIRI）, is the beginning of brain injury, mainly in the mitochondrial structure and function changes. Our previous studies have proved that three active compounds from ethyl acetate extract of Gastrodia elata Blume (EEG) can passed through the brain blood barrier (BBB) and anti-CIRI. The mechanism of anti-CIRI involved in oxidative damage and apoptosis,they are two key aspects of mitochondrial energy metabolism. Suggesting that active compounds can play a role in anti-CIRI by regulating mitochondrial pathways. Furthermore, this project will investigate effects of the three compounds extracted from G. elata exerting on improve mitochondrial dysfunction,maintain the dynamic balance of mitochondrial fusion splitting,activation of mitochondrial autophagy,promote the formation of new mitochondria and interactive regulation of energy metabolism in neurons / astrocytes in CIRI in vitro and in vivo. The active compounds how to regulate the complex energy metabolism network of Mitochondria, and explain the mitochondrial mechanism of active compounds against cerebral ischemia-reperfusion injury.