针刺调控趋化因子FKN/CX3CR1通路抑制视网膜小胶质细胞活性效应研究

感光细胞凋亡是视网膜神经退行性疾病关键致病基础，视网膜小胶质细胞活化是促感光细胞凋亡的重要机制，前期研究证实针刺能抑制感光细胞凋亡、促进视功能恢复，但缺乏针刺对小胶质细胞活性效应的研究，本课题拟以前期证实针刺能抑制感光细胞凋亡为基础，瞄准诱导视网膜小胶质细胞活化、吞噬损伤感光细胞关键通路趋化因子FKN/CX3CR1信号系统，采用ERG、Westernblot、RT-PCR、免疫荧光染色等技术，探索针刺抑制小胶质细胞活性进而阻断感光细胞凋亡进程的确切效应，明确针刺调控趋化因子FKN/CX3CR1信号通路及其下游因子P38、P42、P-SAPK抑制视网膜小胶质细胞活化的关键机制，阐明针刺阻断视网膜感光细胞凋亡进程的科学内涵，获得以视网膜小胶质细胞为作用靶点保护感光细胞的针刺原理和实证依据，建立视网膜感光细胞凋亡的针灸干预评价方法，为针灸防治视网膜退行性疾病研究奠定实验基础，拓展新的研究领域。

The loss of visual function caused by photoreceptor apoptosis is the final outcom in most retinal diseases is inreversible.After photoreceptor cells damaged,retinal microglia are activated,to sawllow, and damage by the chemokines FKN/CX3CR1 pathway accelerate their apoptosis.The topic is based on preliminary results of the study of acupuncture can inhibit the apoptosis of photoreceptor cells ,promote apoptosis of photoreceptor cells and visual function recovery.Intends to use the principle of acupuncture treatment,take chemokines FKN/CX3CR1 as the breakthrough point,use the ERG,Western blot,RT-PCR,immunoflourescence staining and other techniques,to explore the effect of acupuncture on the activity of microglia and the regulation of chemokines FKN/CX3CR1 and the expression of its downstream factors P42,P38,P-SAPK and so on,to clarify the biological effects of acupuncture on the activity of microglia,and its regualation approach and mechanism,to clarify the scientific connotation of acupuncture blocks apoptosis of retinal photoreceptor cells.Obtain empirical evidence of microglia as target to promote the survival of photoreceptor cells.Finally,it provides a new research idea for the prevention and treatment of photoreceptor apoptosis.