稻瘟菌无毒效应因子AvrPii靶调蛋白AVIN6介导的抗病分子机制

稻瘟病是水稻生产中的重要病害。“解析稻瘟菌效应蛋白(Effector proteins)调控水稻抗病信号的功能和作用机制”是认识稻瘟菌致病性和水稻抗病机制的重要科学问题。稻瘟菌无毒效应蛋白AvrPii被抗病蛋白Pii识别激活下游ETI信号，但对于AvrPii在水稻中的靶调蛋白基因的功能和作用机制有待深入研究。本项目以前期利用酵母双杂交筛选鉴定的一个与AvrPii互作的PHD锌指蛋白基因AVIN6以及4个与AVIN6互作的水稻双重特异性蛋白激酶基因OsDPKs为基础，拟研究AVIN6、OsDPKs的抗病生物学功能和作用机制，解析AVIN6、OsDPKs与抗病基因Pii间的遗传互作关系和调控机制，为揭示稻瘟菌效应蛋白调控水稻抗病分子机制提供证据，也为水稻抗病分子育种改良提供理论依据和基因资源。

Rice blast is the most devastating fungal disease for rice. Elucidating the function and molecular basis of Magnaporthe oryzae effectors in rice immune signaling is the critical fundamental scientific project. The blast Avr effector AvrPii has been demonstrated to be perceived by R protein Pii to activate ETI signaling, while how AvrPii modify the rice innate immune signaling remains to be in-depth investigation. In previous study, by yeast-two hybrid screening, we identified one AvrPii-interacting protein AVIN6, and four AVIN6-interacting proteins OsDPKs encoding dual specificity protein kinase. Herein, we proposed to investigate the function and molecular mechanisms of AVIN6, OsDPKs participating in AvrPii/Pii mediated defense signaling. The results of proposed project could shed novel light on understanding the mechanism of M. oryzae effector involved in rice immune modification, and also provide new gene germplasm and strategy for molecular breeding of disease resistance in rice.