外来入侵植物薇甘菊叶抑菌活性成分的分离、结构修饰及其作用机理研究

薇甘菊（Mikania micrantha H.B.K.）为菊科假泽兰属，草本或灌木状攀援藤本植物，原产于中南美洲，是最具入侵性的十大植物之一。上世纪80年代传入我国，给我国广东等地生态及经济带来了极大的危害。然而，薇甘菊在原产地是一种民间广泛使用的草药。本实验室前期的研究发现薇甘菊中含有多种抑菌活性成分，且作用机理独特，但是这些化合物水溶性均很差，严重的制约了其应用价值的开发。故本项目拟1）在前期实验的基础上继续分离薇甘菊中的活性化合物；2）薇甘菊内酯是薇甘菊中含量最多的活性成分，故对其结构进行改造，以期得到易溶于水且活性较好的衍生物；3）去氧薇甘菊内酯为测试药剂，青枯菌为测试菌，从电生理水平、蛋白水平和酶水平阐释去氧薇甘菊内酯对于青枯菌细胞机能影响的作用机制。本项目的完成将为开发薇甘菊为植物源抗生素奠定基础，为从应用的角度去防控入侵植物的为害提供理论依据。

Mikania micrantha H.B.K. (Asteraceae) is a perennial creeping weed. Its native distribution is in tropical central and South America. M. micrantha is listed as one of the world’s worst weeds. M. micrantha are used as a kind of folk medicine in country of origin. So, the aims of this project were 1) based the previous experiment, antimicrobial constituents of M. micrantha will been isolated. 2) The chemical structures of mikanolide will been modified for increasing their water-solubility. This experiment laid the foundation of further development M. micrantha. 3) based the previous experiment, mechanism of deoxymikanolide will be studied about cytomembrane and energy metabolism of Ralstonia solanacearum from the perspective of electrophysiology, protein, nuclear acid, and enzyme levels. In order to through rational exploitation and utilization of M. micrantha, to achieve the purpose of reduce its adverse impact. It is social significant that the result of the project will control alien invasive plant M. micrantha with environmental friendly, rational, sustainable way.