石漠化地区桑树丛枝菌根真菌对宿主根系吸水的促进作用

西南喀斯特石漠化地区植被恢复重建的主要障碍因子是土壤水分的亏缺,然而菌根桑能与此障碍因子相耦合。基于此，本课题以典型的石漠化地区贵州省种植的桑树为研究对象，利用氢、氧稳定同位素技术分析生长于不同喀斯特生境的桑树对不同深度土壤水分的利用比例、利用方式等，探索桑树根系吸水最活跃区和非活跃区；借助形态学方法和现代分子生物学方法即454焦磷酸测序技术对桑树根系吸水最活跃区和非活跃区根内外AM真菌进行鉴定，评价物种多样性，筛选出根内外AM真菌优势菌种；最后通过盆栽实验分析和比较不同接种间和接种与不接种间优势菌种对桑树根系的促水吸收性，为石漠化地区桑树水分高效利用和下一步开展石漠化地区桑树适生机制研究和生态修复工作提供理论支撑。

Soil water deficit is the main obstacle factor of plant reconstruction in the karst rocky desertification area of Southwest China.However,mycorrhizal mulberry could couple with main ecological barriers, that is the seasonal drought,in the rocky desertification area.Therefore,the reasearch takes the mulberry of tapical rocky desertification area in Guizhou Province as a research subject.To begin with,the project,which analyses mublerry grown in different karst habitats to soil water use ratios and ways of water use of different depths by hydrogen and oxygen stable isotope technique,probes the most active and non-active areas of water absorption of mulberry roots.Furthermore,the reasearch assesses species diversity as well as selectes dominant species through identifying arbuscular mycorrhizal fungi of mubelerry roots inside and outside between the most active and non-active areas of water absorption with the aid of morphology method and modern molecular biology method-454 pyrosequencing.Finally,using pot experiments,the reasearch analyses and compares the promoting function of the dominant species to mulberry water absorption bewteen different inoculation and inoculation-noninoculation.To sum up,the reasearch is for the sake of providing theories for water effective use of mulberry,the ecological adaptation of mulberry and the ecological restoration in the rocky desertification area.