低能量激光诱导microRNA调控VEGFA的表达在眼眶新生血管形成的作用及机制研究

新生血管的形成能促进组织再生、创面修复和预防感染，是外科手术成功的关键，血管生成受多种因素的影响，调节机制仍是当前研究的热点。在本课题的前期研究中，我们使用了低能量激光对羟基磷灰石义眼座植入后进行局部照射治疗，在体外和动物模型中均证实了可促进 血管内皮细胞增殖、迁移和成管，并促进义眼座的血管化过程，促进结膜切口的愈合；同时我 们发现在低能量激光疗法处理后有多个miRNAs表达水平的下调，且其中有多个miRNAs可与VEGFA基因的3’-UTR端结合。基于前期实验结果和文献，我们推测低能量激光疗法调节内皮细胞功 能和促进血管生成的作用是通过下调与VEGFA转录相关的miRNAs的表达水平，从而调节VEGFA的转录参与到血管生成的调节过程。为验证上述假设，本课题将深入探讨低能量激光疗法诱导下 miRNAs-VEGFA调节途径对血管生成的作用机制。将为今后治疗血管生成依赖性疾病提供新的治疗靶点。

The Neovascularization can promote tissue regeneration, wound to repair and prevent infection, The key to the success of surgery. Angiogenesis is affected by a variety of factors, the relevant mechanism is still a current researching focus. In the previous research of this topic, we used a low-level laser to irradiation hydroxyapatite implanted after local implantation. Both in vitro and animal models have confirmed that it can promote the proliferation, migration and tube formation of vascular endothelial cells, promoting the vascularization of the hydroxyapatite impalnts and the healing of conjunctival incisions .We also found that there are several miRNAs down-regulated after low-level laser treatment, and there are multiple miRNAs that can bind to the 3'-UTR end of the VEGFA gene. Based on previous experimental results and literature, we speculate that low-energy laser therapy regulates endothelial cell function and promotes angiogenesis by down-regulating the expression of miRNAs related to VEGFA transcription in cells, thereby regulating VEGFA's transcription and regulating angiogenesis. Process to verify the above hypothesis, this topic will further to investigate the mechanism of angiogenesis induced by low-energy laser therapy induced by miRNA-VEGFA. Therefore, this topic will provide new therapeutic targets for the treatment of angiogenesis-dependent diseases in the future.