从二仙汤配伍研究补肾法对乳腺增生症雌孕激素受体通路的调节

本课题在已结题课题基础上，选用临床疗效确切且具有前期实验基础的二仙汤作为补肾治疗乳腺增生症的代表方，以方代法，拟从体内、体外实验两部分探讨二仙汤全方及其拆方：补肾阳方（仙茅、仙灵脾、巴戟天、当归）、滋肾阴方（知母、黄柏）的治疗作用及机制。从乳腺体积、腺体厚度、乳腺上皮细胞增殖情况分别观察二仙汤及其拆方的治疗效应，从激素水平变化、激素受体调节、受体反应元件、辅激活因子、辅抑制因子的变化切入，观察补肾法及其拆方对雌、孕激素受体信号传导通路的调节作用。通过整体、组织水平、细胞水平、分子水平等多层次的研究，探讨二仙汤的组方机理，揭示补肾法对乳腺增生症内分泌紊乱的调节机制，以期为从补肾法论治乳腺增生症奠定实验基础,丰富方剂组方理论的科学内涵。

This project is based on a completed funding. We select erxian decoction which has good clinical effect and experimental basis to treat mammary gland hyperplasia as kidney invigorating method representative. The research including in vitro and in vivo experiments is to explore erxian decoction and its decomposed part effect and mechanism. The Kidney Yang invigorating decoction includes Curculigo, Epimedium , Morinda and Angelica, Kidney Yin nourishing decoction includes Anemarrhena, Cork. The effect is studied from breast size, thickness, mammary epithelial cell proliferation by different kidney invigorating method. The mechanism is studied from changes in hormone levels, hormone receptor modulators, receptor response elements, coactivators and coinhibitors. This research includes tissue level, cellular level and molecular level. The purpose is to reveal the mechanism of kidney invigorating method on endocrine disorder of mammary gland hyperplasia.It can offer guideline to the clinic. To investigate the mechanism of erxian decoction can enrich decoction components theory.