基于血清谱效学和PK-PD模型的苗药云实皮抗炎药效物质基础研究

云实皮为贵州苗族常用药材，对于支气管炎、流行性感冒的治疗有独特疗效，已有多个民族药品种上市。课题组首次研究发现云实皮乙醇提取物的水溶性部位具有显著的抗炎作用（首次分离得到11个化合物），但其药效物质基础、作用机制及体内过程尚不明确。本研究旨在以云实皮有效组分为研究对象，以血清谱效学为手段，以UPLC/Q-TOF MS技术建立指纹图谱，全面分析云实皮入血成分，基于炎症模型探讨体内移行成分与抗炎活性的相关性，从病理状态和细胞药代动力学的角度，建立PK-PD模型，开展云实皮在炎症疾病动物模型和细胞模型下的药代动力学和抗炎作用相关性研究（即药物浓度-时间-效应关系），揭示其抗炎作用的效应成分及体内动态变化规律，最终阐明云实皮抗炎作用的物质基础，为其深层次开发利用奠定理论和试验基础。

Caesalpinia decapetala（Roxb.）Alston, a Miao national herb in Guizhou, is commonly used for treatment and prevention of bronchitis and influenza, and a number of listed ethnic preparations contain C. decapetala. Although its chemical components and pharmacological activity have been reported, its anti-inflammatory therapeutic material basis and mechanism remain unclear. In our previous study, it was the first time to found that the water soluble fraction, of which 11 were found from this species for the first time, also had a significant effect on the inflammatory model. Based on the previous systematic research of chemical components, pharmacological action and effective soluble fraction, the project will focus on those migrating into the blood by means of serum spectrum-activity analysis and the fingerprint of UPLC/Q-TOF MS technology. Furthermore, the potential relevance between these constituents and pharmacological action will be put into the study to explore the anti-inflammation ones, using inflammatory models. Thereafter, the serial research will be conducted on ADME and anti-inflammation test by PK markers, using animal disease model and cell model, so as to build the PK-PD model which may demonstrate the action between the multicomponents and the pharmacological activities (Aiming at the relevance of the Concentration, Time and Effectiveness). Through the metabolism fate study of the anti-inflammation effective constituents and their dynamic transformation in vivo, it can help find out the therapeutic material basis in the herb and to lay a theoretical and experimental foundation for further development and application of the medicinal plant.