性激素α1诱导疫霉菌有性生殖细胞信号转导通路的蛋白质组学研究

疫霉菌是一类毁灭性植物病原菌，其有性生殖对物种演化和延续起着重要作用。近期，调控疫霉菌有性生殖的性激素α1已被鉴定，但其调控的有性生殖信号转导通路至今尚无报道。.在后基因组时代，快速、高效、系统揭示细胞信号转导通路分子机理已成为可能。本研究拟以致病疫霉为研究对象，① 通过比较蛋白质组学、磷酸化蛋白质组学等方法，研究疫霉菌性激素α1调控的有性生殖过程中蛋白质组、磷酸化蛋白质组的动态变化，以确定该通路成员。② 通过RNA干扰技术对一些重要通路成员的功能进行鉴定。③ 通过生物信息学工具对差异表达基因进行基因注释、相互作用网络等分析，初步建立疫霉菌性激素α1调控的有性生殖细胞信号转导通路。.该研究将为揭示疫霉菌性激素α1调控的有性生殖细胞信号转导通路分子机理奠定基础，将可能为植物疫病的有效防治提供新思路和重要理论依据。

Phytophthora is a genus of highly destructive plant pathogens, whose sexual reproduction plays an important role in evolution and continuation of the species. Recently, the hormone α1 regulating the sexual reproduction of Phytophthora has been identified, but its signal transduction pathway has not been reported..In the post-genomic era, it has become possible to discover the molecular mechanism of cellular signal transduction pathways rapidly, efficiently and systematically. Therefore, in this study, we are going to do the following research on Phytophthora infestans: ① determine the members involved in the sexual reproduction regulated by hormone α1 in Phytophthora by studying the dynamic changes of proteome and phosphoproteome in the process of sexual reproduction in Phytophthora by comparative proteomics and phosphoproteomics methods. ② identify the function of the crucial members by RNA interference technology. ③ construct the signal transduction pathway regulated by hormone α1 in sexual reproduction of Phytophthora by the bioinformatics analysis. .Our study will lay a foundation for revealing the molecular mechanism of signal transduction pathway involved in sexual reproduction regulated by hormone α1 in Phytophthora and may provide new ideas and important theoretical basis for the effective prevention and treatment of plant diseases caused by Phytophthora pathogens.