miR-128靶向GAREM调控ERK/MAPK信号通路影响牦牛子宫内膜炎症损伤的分子机制

子宫内膜炎是影响牦牛产业发展的重要疾病之一。我们前期研究发现：MAPKs信号通路关键因子在牦牛子宫内膜炎症损伤过程中存在差异性表达；同时，我们通过荧光定量RT-PCR证实miR-128及其靶基因GAREM在牦牛子宫内膜炎症损伤过程中表达异常。那么牦牛子宫内膜炎发病过程中，miR-128是否靶向GAREM调控ERK/MAPK信号通路影响炎症的发生？机制是什么？目前尚无报道。为此，本项目拟以牦牛子宫内膜上皮细胞为模型，通过miR-128及其靶基因GAREM过表达或干扰、荧光定量RT-PCR及Western blot等方法，研究miR-128及GAREM表达对LPS诱导牦牛子宫内膜炎症损伤的影响，以及miR-128在ERK/MAPK信号通路中所发挥的调控作用，阐明miR-128通过靶向GAREM调控ERK/MAPK信号通路影响牦牛子宫内膜炎症损伤的分子机制，为寻找相关治疗靶点提供理论依据。

Endometritis is one of the common postpartum disease in yaks, which seriously affects the reproductive performance. Our previous study found that the key factors of MAPKs signaling pathway are differently expressed in LPS induced inflammatory injury endometrium in Yaks; Meanwhile, we confirmed the abnormal expression of miR-128 and its target gene GAREM in LPS induced endometritis in Yaks by qRT-PCR. However, does miR-128 on endometritis in Yaks can target GAREM to regulate ERK/MAPK signaling pathway is still unknown, and what is the molecular mechanism? The relevant regulatory mechanism is still unclear. For these purpose, the endometrial epithelial cells from Yaks were cultured, and overexpression or silencing of miR-128 and GAREM, qRT-PCR and western blot were used to investigate the effects of miR-128 and GAREM expression, and regulatory role of miR-128 in ERK/MAPK signaling pathway on LPS-induced endometrial inflammatory injury in yaks. To further elucidate the molecular mechanism of miR-128 on inflammatory injury of LPS induced endometritis by targeting GAREM mediated ERK/MAPK signaling pathway, which will provide a theoretical basis for further revealing the therapeutic targets of endometritis in Yaks.