内蒙古粘细菌资源多样性及其抗马铃薯晚疫病活性分析

粘细菌是一类具有极大应用研究开发潜力的微生物类群。前期研究表明，内蒙古呼和浩特地区分离的34株粘细菌中，有30株表现出了对马铃薯晚疫病菌-致病疫霉的明显抗性。 鉴于内蒙古地区从东到西的不同生态环境条件，本研究拟从内蒙古东部到西部地区采集代表性土壤、植物、水样等样品：① 分析内蒙古地区粘细菌资源的多样性。测定采集样品环境参数，通过PCR-DGGE技术分析粘细菌非培养多样性，对样品进行粘细菌分离鉴定，分析粘细菌可培养多样性。分析粘细菌分布与环境参数间的相关性。② 分析粘细菌的抗马铃薯晚疫病活性。测定菌株抗致病疫霉活性，对具有高抗活性的粘细菌进行活性物质的分离鉴定，通过测定活性物质对致病疫霉菌丝体、孢子形态、细胞壁、细胞膜、无性生殖、有性生殖等的影响进行作用机理研究，分析活性物质的盆栽温室防效与安全性。 该研究可能为丰富我国粘细菌资源及研发有效防治马铃薯晚疫病的生物农药做出一定贡献。

Myxobacteria are a group of microbe resources with great potential for the applied research and development. Our previous study showed that 30 of the 34 myxobacteria strains separated from some samples in Hohhot in Inner Mongolia exhibited significant resistance to the pathogen of potato late blight - Phytophthora infestans. In view of the different ecological conditions of Inner Mongolia from east to west, we are going to collect the representative soil, plants, water samples from the eastern region of Inner Mongolia to the western region to do the following research: ① Analyzing the diversity of myxobacteria resources of Inner Mongolia. The collected samples are measured the environmental parameters. The diversity of myxobacteria is analyzed by PCR-DGGE method and culture-dependent approach. At last, the correlation between the myxobacteria distribution and environmental parameters are anayzed. ② Analyzing the resistance of the myxobacteria strains to potato late blight. The resistance of the strains to Phytophthora infestans are determined. The active substances of the strains with high resistance are separated and identified. The action mechanisms of the active substances are analyzed by observing the impact of the substances on mycelia, spore morphology, cell wall, cell membrane, asexual reproduction, sexual reproduction of Phytophthora infestans, etc. Moreover, the control effects of the active substances on potato late blight in greenhouse and their application safety are analyzed. This study may make some contribution to enrich the myxobacteria resources of our country and develop new biological pesticides for the effective prevention and treatment of potato late blight.