邻苯二甲酸二丁酯（DBP）降解菌的筛选及其生物降解途径研究

邻苯二甲酸二丁酯（DBP）是一种重要的酞酸酯（PAEs）类化合物，具有很高的生物毒性，是塑料棚膜和农药的重要成分，目前农业用地中 DBP 残留问题已成为全球普遍关注的热点问题。然而由于受污染物农业用地中 DBP 分布不均及传质效率低等局限，土壤中高效 DBP 降解菌进化缓慢，有关 DBP 的降解机理及功能基因的研究鲜有报道。为全面认识功能菌对 DBP 降解调控机制，提高修复效率，本项目拟通过培养驯化富集的方式，获得一株 DBP 高效降解菌并系统研究其降解特性，进而全面获取 DBP 的生物学降解动力学特性及降解菌体微观动态变化；通过转录组学、荧光定量 PCR 技术、异源表达技术及相关蛋白的酶学特性研究，系统剖析 DBP 的生物降解途径，系统揭示功能基因对 DBP 代谢的调控机理。研究成果为解决 DBP 生物代谢调控机制研究提供理论基础，有望为农业 DBP 污染生物修复的研究打开新思路。

Di-n-butyl phthalate (DBP) as an important compound of phthalic acid esters (PAEs) has a higher biological toxicity and is widely used in the plastic and pesticide manufacturing industries. The continuous use of agricultural films and insecticide can involve risks and hazards to humans, animals and the environment. Although the problems of DBP residues in agricultural land have become an issue of major concern in current studies, DBP-degrading bacteria resources evolve slowly due to the uneven distribution of the contaminant and lower mass transfer efficiency in polluted farm soil. And the researches to explicate proper understanding of DBP degradation mechanisms are still rare. This project therefore aims to screen and identify degrading strains by enrichment culture and illuminate the degradation characteristics, DBP biological degradation kinetics and dynamic changes assessment of degrading strains. Incorporated with transcriptomics, Quantitative Real-time PCR, heterologous expression of hypothetic genes and enzymatic properties, the correlation among the DBP metabolic network, biodegradation pathway and regulation mechanism will be detected. This research would provide the theoretical evidence to determine the DBP biodegradation pathway, molecular mechanisms, and promote a new perspective on ecological remediation of DBP contamination in agriculture.