纤维素酶处理活化溶解浆的作用机理及其强化机制

纤维素分子的活化处理是提高溶解浆在下游产业中应用价值的重要步骤，而采用生物酶代替化学品的活化技术是改善生产/生活环境的关键，具有十分重要的战略意义。本项目拟以预水解硫酸盐法溶解浆为研究对象，采用内切酶为主的纤维素酶对其进行活化、修饰处理，深入研究纤维素在酶活化过程中的解聚和微观结构变化规律，进而揭示纤维素与酶活化作用间的构效关系；并通过分析大量的酶处理样品探明纤维素宏观指标的变化规律，建立粘度和反应性能这两项重要指标之间的映射关系；综合纤维底物因素和酶相关因素两个方面来研究溶解浆酶法活化过程的强化机制，通过分析底物可及性和酶蛋白吸附率的变化规律，进一步弄清物理处理、化学处理以及两者协同处理对强化纤维素酶活化过程的作用途径。本项目的完成将为溶解浆清洁化生产及高值化利用奠定坚实的科学基础，具有十分重要的现实意义和理论价值。

Cellulose activation of dissolving pulp is the prerequisite for its downstream industrialized application. For such purpose, using green bio-technology instead of chemical treatment is the key for improving production and living environment, which is of strategic significance. Therefore, the pre-hydrolysis kraft based dissolving pulp was selected as raw material in this proposal, and the endoglucanase-rich cellulase was used to activate and modify the cellulose. .Firstly, the cellulose degradation rule of cellulase activation process was investigated to reveal the response mechanism of cellulose during enzymatic hydrolysis. Subsequently, the two key parameters of viscosity and reactivity under various cellulase treatment conditions were determined to build samples database. Based on the database, the corresponding mathematical equations could be developing. Finally, the technology of mechanical or chemical or those synergy effects on enhancing enzymatic efficiency was explored from both cellulosic substrate and enzyme related factors. Furthermore, the enhancing mechanism could be clarified through the substrate accessibility and cellulase adsorption. The completion of the project would be great significance in fostering the successful commercialization of cleaning production and value-added application of dissolving pulp.