微管相关蛋白AtMAP65-8调控微管动态响应渗透胁迫的分子机制

植物中的微管相关蛋白通过调控微管的动态变化，参与植物响应逆境胁迫。MAP65蛋白家族是一类相对保守的微管结合蛋白，在拟南芥中共有9个成员，目前多数蛋白定位已报道，但是具体功能研究的较少。前期通过RNA-Seq数据筛选出MAP65-8能够被盐胁迫显著诱导表达，通过表型分析发现MAP65-8参与植物响应渗透胁迫过程。因此，本项目主要研究MAP65-8在植物响应渗透胁迫中的作用，并探究其分子机制。进一步研究揭示MAP65-8是否通过调控微管的动态变化响应渗透胁迫。此外，前期结果表明MPK4与MAP65-8蛋白互作，进一步研究将揭示MPK4如何调控MAP65-8蛋白功能，进而影响微管的动态不稳定性来响应渗透胁迫。本项目的实施，将进一步解析植物响应渗透胁迫的分子机理，为增强植物抗逆提供理论依据。

Microtubule-associated Proteins (MAPs) regulate the dynamic instability of microtubules in response to abiotic stresses in plants. MAP65 proteins are relative conservative MAPs and nine members of the MAP65 protein family have been identified in Arabidopsis. Characterizing the locations of MAP65s has been mostly reported, however, the functions are not well understood. In previous study, MAP65-8 was identified from RNA-Seq data, which is induced by salt stress. Nevertheless, the phenotype experiments indicated that MAP65-8 is involved in response to osmotic stress. Therefore, this project intends to study the function of MAP65-8 in response to osmotic stress and further explore its molecular mechanism. Further studies will reveal whether MAP65-8 regulates the dynamic instability of microtubules in response to osmotic stress. Moreover, the previous results indicated that MPK4 interacts with MAP65-8, and further studies will reveal how MPK4 regulates the function of MAP65-8, thereby affecting the dynamics instability of microtubules in response to osmotic stress. This study will further reveal the molecular mechanisms of plant responding to osmotic stress, and provide a theoretical basis for enhancing plant stress resistance.