牦牛不同来源胚胎早期发育过程中LncRNAs介导Paraspeckle形成对细胞命运决定的调控机制

核旁斑点(Paraspeckles)作为特殊细胞核结构，参与细胞核染色质重组、组蛋白修饰等多种生理应答。初步证实，CARM1、NEAT1与Paraspeckles结合在小鼠早期胚胎发育中决定细胞命运，但对其他哺乳动物胚胎发育的生理作用未见详细研究，在胚胎不同基因激活方式中的差异仍需探索。项目拟以高原牦牛为研究对象，利用单细胞测序技术分析不同来源胚胎早期发育过程中LncRNA差异，评估Paraspeckles，筛选与Paraspeckles形成相关LncRNA；调控LncRNA，分析对Paraspeckles形成的影响，评估胚胎发育能力和囊胚细胞分布；检测LncRNA对CARM1、组蛋白修饰及细胞命运决定因子的调控，阐明LncRNAs介导Paraspeckle形成在牦牛不同来源胚胎早期发育过程中对细胞命运决定的调控机制。项目有助于改善牦牛体外胚胎的发育和附植能力，提高牦牛繁殖效率和经济效益。

Paraspeckles are one of cell subnuclear bodies and identified recently, which respond dynamically to a variety of basic physiological processes such as nuclear repositioning, reorganization of chromatin and histone modifications. It has been reported that the association of CARM1, NEAT1 with paraspeckles in 2- to 4-cell stage mouse embryos impacts proper lineage allocation. However, its potential effect has never been carefully investigation during pre-implantation development in other mammalians, which also need to be confirmed in embryos produced by different methods. The yak will be as object of study in this project, and the difference of paraspeckles and LncRNAs were analyzed at early stages of embryos produced by different methods, selective LncRNAs that related to paraspeckles formation. The influence of LncRNAs on paraspeckles formation, pre-implantation development and cell lineages in blastocyst will be examined by detected the levels of LncRNAs during early mammalian development. The regulation of LncRNAs on pluripotency factors, histone modifications and CARM1 will also be detected, and used for to clarify the signal of paraspeckle and LncRNAs regulate cell fate in yak pre-implantation embryo produced by different method. This project will be help to enhance the development and implantation of yak embryos, and improve economic benefits and reproduction efficiency in yak.