大豆种子光泽度基因B2的克隆和功能分析

大豆是重要的粮食和经济作物，种子光泽度是大豆商业价值的重要体现并影响种子品质的形成。本研究采用图位克隆的策略，利用我国东北和黄淮海大豆主产区10个品种构建的5套遗传分离大群体，精细定位和克隆控制栽培大豆种子光泽差异的关键基因B2，并利用大豆种质资源解析B2基因的驯化特点并探索HPS基因簇的功能，建立大豆种皮光泽与大豆种子品质内在联系，并运用分子生物学和生物信息学技术，筛选和鉴定B2的互作蛋白，系统解析B2基因参与的大豆种子光泽调控的分子机制，为大豆品质改良提供新思路和新靶点。

Soybean is an important source of food and economic crop. Seed coat luster of soybean reflects the commercial value of soybean and affects the formation of the seed quality. In this project, five recombinant inbred line populations developed by the crosses between 10 varieties of soybean in Northeast China and Huang-Huai-Hai region are used to isolate and clone the gene B2 by map-based cloning. Soybean germplasm resources are used to analysis the domestication characteristics of the gene B2 and explore the function of the HPS gene cluster and establish the relationship between soybean seed coat luster and seed quality. We screen and identify B2 interaction protein with the confirmation of molecular biology and bioinformatics and elucidate the molecular mechanism of seed coat luster formation in soybean. This research will provide new ideas and information in improving quality of soybean.