从TRPV1/TAS2R调节钙稳态平衡探讨射干麻黄汤宣降相因对支气管哮喘的调控机制

细胞内钙离子稳态失衡会加重哮喘的进展，瞬时受体电位香草酸亚型1（TRPV1）和II型味觉受体（TAS2R）在哮喘中高表达，会引起细胞内钙稳态的失衡。支气管哮喘属中医“哮病”，主要病机为肺气宣降失衡，“苦泄降肺，辛散宣肺”为哮病主要治法，TAS2R在味蕾中介导苦味味觉的产生，辛味药性主要是通过TRPV1发挥药效，而射干麻黄汤是辛宣苦泄治疗寒哮的经典方。基于此，我们提出假说：射干麻黄汤通过TRPV1/TAS2R调节钙稳态发挥辛宣苦泄的功效来调衡肺气宣降以治疗哮喘。本项目通过体内和体外研究验证该假说，其结果将为哮喘辛宣苦泄治法提供理论依据，丰富“肺主宣降”理论的内涵，并且为创制新方治疗哮喘提供机制参考。

The imbalance of intracellular calcium homeostasis will aggravate the progress of asthma. The overexpression of TRPV1 and TAS2R in asthma will lead to the imbalance of intracellular calcium homeostasis. Bronchial asthma belongs to asthma of Traditional Chinese Medicine, the main pathogenesis is the imbalance of Lung Qi's dispersing outwards and inwards, and the main treatment of asthma is "bitter disperses inwards Lung Qi, and pungency disperses outwards Lung Qi". TAS2R mediates the generation of bitter taste in taste buds. The medicine of pungent taste mainly exerts its effect through TRPV1, while Shegan Mahuang Decoction is the classic prescription of bitter disperses inwards and pungency disperses outwards Lung Qi, and treating cold asthma. Based on this, we put forward the hypothesis that Shegan Mahuang Decoction can regulate the balance and reduce the pressure of asthma by regulating the calcium homeostasis and exerting the effect of bitter disperses inwards and pungency disperses outwards Lung Qi through TRPV1 / TAS2R. This project verifies the hypothesis through in vivo and in vitro research, and the results will provide theoretical basis for the treatment of asthma, enrich the connotation of the theory of "lung controlling dispersing outwards and inwards", and provide mechanism reference for the creation of new prescriptions for the treatment of asthma.