草莓果实成熟相关泛素连接酶E3的鉴定及功能分析

草莓果实含有丰富的营养物质，深受消费者喜爱。研究草莓果实成熟调控机制对于提升果实品质和延长果实采后保鲜时间具有重要意义。草莓作为典型的非呼吸跃变型果实，其成熟过程受到体内、体外诸多因子的调控。目前，关于草莓果实的成熟调控主要集中在转录调控上，而对于转录后调控，尤其是翻译后修饰在草莓果实成熟调控中的作用还未见系统报道。泛素化是蛋白质翻译后修饰的重要形式，对蛋白质稳定性、定位和功能等具有重要作用。本研究拟在前期研究的基础上，以草莓果实为材料，利用蛋白质组学、生物化学和分子生物学，以及反向遗传学等方法，鉴定在草莓果实成熟过程中发生泛素化修饰的蛋白质，分析催化这些蛋白质发生泛素化修饰的泛素连接酶，并解析它们的生物学功能，从而揭示蛋白质泛素化调控草莓果实成熟的分子机制，为全面认知草莓果实成熟和品质调控的分子网络提供新证据。

Strawberry contains plenty of nutrients and is one of most popular fruits. It is important to study the regulatory mechanisms of strawberry ripening for improving fruit quality and prolonging post-harvest storage time. Strawberry is a typical non-climacteric fruit, with its ripening process tightly controlled by various complex intrinsic signal and environmental factors. Previous researches on strawberry ripening have been more focused on transcriptional level, but the post-translational regulation of fruit ripening remains elusive. In particular, the regulatory roles of ubiquitination in fruit ripening are largely unknown. Protein ubiquitination is one of the most important post-translational modifications, plays important role in protein function, and involves in many cellular processes. By using approaches in proteomics, biochemistry, molecular biology, and reverse genetics, we aim to identify the ubiquitiated proteins associated with strawberry fruit ripening. The ubiquitin ligases (E3s) involved in ubiquitination of those ripening proteins will be characterized. These works will provide theory basis for engineering technology to control ripening or maintain postharvest quality of strawberry.