泡核桃黄酮醇合成酶基因JsFLSs克隆及功能验证

泡核桃是原产我国西南地区的优异的药食两用核桃资源，黄酮醇芦丁是其主要的功能物质，对人类健康具有重要作用。但目前泡核桃高含量芦丁积累的原因仍不甚明了，阻碍了泡核桃种仁内芦丁含量的定向改良。本项目克隆黄酮醇合酶基因（JsFLS）家族成员，研究泡核桃芦丁合成代谢中各JsFLSs家族成员的作用，明确JsFLSs蛋白最适底物及其催化动力学参数，解析黄酮醇合成代谢流的流向和强度，阐明JsFLSs基因表达与芦丁合成的关系。通过本项目的研究，可望获得JsFLSs家族成员基因全长，阐释JsFLSs家族基因在泡核桃芦丁合成中的功能，为核桃栽培技术调控黄酮醇合成提供理论支撑，也为核桃品质分子育种奠定基础。因此，具有重要的科学意义和应用价值。

Juglans sigillata is a kind of outstanding walnut resources with medical and edible functions, which originated from the southwest of China. The rutin, which belong to flavonol, is the main functional substance and is very important for human healthy. However, the reason of high content of rutin in J. sigillata is still unclear, which hinders the directional improvement of rutin content in walnut seed kernel. The aim of this project is to clone the members of flavonol synthetase (JsFLS) gene family, study the function of each member of JsFLSs in rutin biosynthesis metabolism of walnut, identify the most suitable substrate and its catalytic kinetic parameters of JsFLSs protein, and elucidate the relationship between the expression of JsFLSs gene and rutin biosynthesis by analyzing the flow and intensity of flavonol biosynthesis. Through this project, we hope to obtain the full length of JsFLSs family genes, explain the function of JsFLSs family genes in rutin synthesis of J. sigillata. The project will not only provide theoretical support for the regulation of flavonol synthesis by cultivation techniques of J. sigillata, but also lay a foundation for molecular breeding of walnut quality. Therefore, it has important scientific significance and application value.