基于结构变异的大熊猫比较基因组学与种群基因组学研究

以往的大熊猫种群基因组学和比较基因组学研究，探索了大熊猫种群单核苷酸变异（SNP）的分布规律，并从SNP的角度阐释了部分适应性性状产生的潜在遗传机制，但尚未挖掘结构变异（SV）的组成和分布规律，及其与大熊猫性状的潜在联系。本研究拟通过基于三代测序数据的大熊猫基因组序列数据与其它近缘物种高质量基因组序列比较，挖掘大熊猫种间特有SV；利用大熊猫三代、二代重测序数据，挖掘大熊猫种群内SV，并基于获取的大熊猫SV，进行比较基因组学与种群基因组学研究。研究旨在掌握大熊猫种间与种群SV的组成及分布规律，探讨种间与种群SV与大熊猫性状间联系。研究将从SV角度为野生大熊猫进化与保护提供进一步的认识；将建立基于SV的野生动物比较基因组学和种群基因组学研究模式，为其它野生动物的SV研究提供参考和借鉴。

Previous research explored the distribution of SNPs in the giant panda population, and explained the potential genetic mechanisms of some adaptive traits from the perspective of SNP, but did not explore the composition and distribution of structural variation (SV), and its potential impacts. This research intends to use the giant panda genome sequence based on the third-generation sequencing data to compare with the high-quality genomes from the other closely related species to mine the panda-specific SVs, uses the third- and second-generation giant panda resequencing data to mine the SVs between panda populations, and performs comparative genomics and population genomics analyses based on the obtained SVs. The research aims to grasp the composition and distribution of SV among species and between panda populations, and to explore the relationship between SV and important traits of giant pandas. The research will provide further insights into the evolution and protection of wild giant pandas from the perspective of SV, it will also provide SV-based comparative genomics and population genomics research models for other wild animals.