北部湾海水养殖鱼类弧菌病流行规律、病原诊断及致病机理研究

弧菌病是海水鱼类养殖中最严重的病害之一，给我国海洋经济造成巨大经济损失。申请人在前期工作中对湛江沿海主要海水养殖区弧菌病进行流行病学调查及分子流行病学分析，证实该海域弧菌病暴发与海水理化因子、异养菌数量、弧菌数量及病原弧菌分泌的毒力蛋白具有关联。本项目拟通过北部湾沿海主要海水养殖鱼类主养区弧菌病病因调查、建立弧菌病原库，全年监控养殖海区环境因子的变化，寻求养殖环境因子与弧菌病发生与流行的相互关系，分析弧菌病的流行规律；从组学水平探讨不同来源菌株之间弧菌毒力因子的表达差异，分析海水养殖鱼类弧菌病的分子流行机制；开发基于特定毒力因子的分子生物学快速诊断技术；筛选和研究T3SS在弧菌不同菌株中的分布及其效应蛋白、生物学功能和T3SS效应蛋白与宿主蛋白的互作对感染鱼类细胞的影响，从而阐明弧菌病原侵染宿主的致病机制。研究结果为有效防控海水养殖鱼类弧菌病的发生与流行提供科学依据。

Vibriosis is one of the most serious diseases in marine culturing fish, and has caused huge economic losses to China's marine economy. The epidemiological survey and molecular epidemiological analysis of Vibriosis in the main marine aquaculture area of the coast of Zhanjiang was performed in the preliminary work, and the relationship among the outbreak of vibriosis and the chemical factors of water, the number of heterotrophic bacteria, the number of Vibrio and the virulence proteins secreted by Vibrio was found. In this work, based on the preliminary study, we intend to monitor the changes of environmental factors in the cultured sea area throughout the year, investigate the etiology of vibriosis in the main marine aquaculture fish culture zone in the Beibu Gulf Coast, and seek the interaction between the environmental factors and the occurrence and prevalence of vibriosis. We also will explore the expression differences of vibrio virulence factors under different environmental conditions and strains from different sources, analyze the molecular epidemic mechanism of vibriosis in marine aquaculture fish, and develop molecular biology diagnostic techniques based on specific virulent genes. Furthermore, we will screen and study the distribution of T3SS in different strains of Vibrio and its effector proteins, biological functions, and the interaction of T3SS effector proteins with host proteins of infected fish cells. The results will clarify the pathogenic mechanism of Vibrio pathogens infecting the host, and provide a scientific basis for the effective prevention and control of the occurrence and prevalence of vibriosis in marine aquaculture fish.