三七总皂苷调控NF-κB/HIF-1α/VEGF通路干预COPD大鼠肺血管重构的作用机理研究

慢性阻塞性肺疾病（COPD）是以气道炎症、重塑和肺血管重构为基本病变慢性肺部疾病，已成为全球第三大死因，我国患病人数已超1亿。GOLD以糖皮质激素抗炎和支气管舒张剂解痉为主的治疗方案，能缓解患者症状但不能阻止肺部病变进展和肺功能持续下降的趋势，而辅以中医益气涤痰逐瘀的治法，是减缓病变持续发展的有效策略。课题组前期研究已证实云南省名中医的益肺散结验方及方中的三七能通过抗炎、抗纤维化作用改善COPD病变，但药物对肺血管重构作用尚未深入研究，云南地处高原，改善血瘀状态对缓解患者低氧血症、提高生活质量意义重大。本课题拟以三七有效成分三七总皂苷干预COPD大鼠，观察药物对大鼠肺功能、肺部炎症和肺血管重构病变的改善作用以及其对肺部炎症因子表达和NF-κB/HIF-1α/VEGF通路相关分子的调控规律，揭示PNS干预肺血管重构的可能分子机制，为活血化瘀药物防治COPD提供理论和实验依据。

Chronic obstructive pulmonary disease (COPD) is a chronic lung disease characterized by airway inflammation, remodeling and pulmonary vascular remodeling as the basic lesions and continuous airflow limitation. It has become the third leading cause of death worldwide. Over 100 million in our country . GOLD is mainly based on anti-inflammatory glucocorticoids and antispasmodic bronchodilators, which can relieve patients' symptoms but cannot prevent the progress of lung lesions and the continuous decline of lung function. Supplemented by Chinese medicine yiqi ditan zhuyu therapy , is an effective strategy to slow down the continuous development of lesions. The preliminary research of the research group has confirmed that the Huoxue drug Sanqi in the Yifei Sanjie prescription ,which from Yunnan famous TCM doctor,can improve COPD lesions through anti-inflammatory and anti-fibrotic effects, but the effect of intervention on pulmonary vascular remodeling has not been studied in depth. Being on a plateau, improving blood stasis is of great significance to relieve patients with hypoxemia and improve quality of life. Therefore, we will intend to intervene COPD rats with Panax notoginseng, the main active ingredient of Panax notoginseng, to observe the improvement effect of drugs on lung function, pulmonary inflammation and pulmonary vascular remodeling lesions in rats, as well as its expression of inflammatory factors and NF -κB / HIF-1α / VEGF pathway related molecular regulation rules, revealing the possible molecular mechanism of PNS intervention in pulmonary vascular remodeling, providing theoretical and experimental basis for the treatment of COPD with blood circulation and blood stasis drugs.