中医㨰法样刺激对损伤骨骼肌细胞机械敏感通路触发机制研究

体内细胞如何识别手法作用力的改变，并将手法特有的力学信号转变为电生理及化学信号，进而引起一系列生物学效应，是阐述手法作用机制的关键环节。本项目将联合美国北德克萨斯大学健康科学中心，通过Flexcell细胞力学加载系统开展㨰法样刺激对损伤骨骼肌细胞机械敏感通路触发机制研究。应用高通量转录组学与蛋白组学联合技术及膜片钳技术分别筛选力感受器途径和力敏感离子通道途径；重新培养损伤骨骼肌细胞，对筛选出的可能传导通路加以阻断，在力学加载后检测骨骼肌细胞凋亡、超微结构及肌酸磷酸激酶等代谢指标的应答状况，探究该通路是否为㨰法样刺激由力学信号转换为电生理及化学信号的转导途径。该研究将阐释推拿手法生物学效应启动环节的信号转导规律，为推拿手法的作用机制提供理论依据和实践资料。

It is a key link in the study of manipulation mechanism how the cells in the body identify the change of manipulation force, and then translate these characteristic mechanics signal into the physical and chemical signal which results in a series of biological effects. Collaborating with University of North Texas Health Science Center, the project will study on trigger mechanism of mechanical sensitive pathway of injured skeletal muscle cells by Chinese rolling manipulation-like stimulation with the Flexcell cellular mechanical loading system. The force receptor pathway and force sensitive ion channel way will be screened by the techniques of high throughput transcriptome and proteomics and patch clamp respectively. After the Flexcell cellular mechanical loading, the skeletal muscle apoptosis, ultrastructure and metabolic indicators such as CK will be tested after the injured skeletal muscle cells are loaded the mechanical stimulation imitating Rolling Manipulation when the possible screened pathways are blocked, which verify the validity of transduction pathways converting mechanical signal to electrical physiological and chemical signal.The study will explain the signal transduction law initiating the biological effect of Chinese manipulation, which provides the theoretical basis and practical information for the mechanism of manipulation in Traditional Chinese Medicine.