罗非鱼NCC表面凝聚素受体的鉴定与功能研究

鱼类非特异性细胞毒性细胞（Nonspecific cytotoxic cell，NCC）的功能相似于高等脊椎动物的自然杀伤细胞（Natural Killer Cell，NK）。NK细胞表面受体分子类型、功能、活性调控业已研究清楚。但除NCCRP-1外，NCC表面其它受体分子的研究尚为鲜见，制约了对NCC功能及其活性调控的全面认知。因此，本课题应用单细胞RNA测序（scRNA-seq）技术获得了罗非鱼头肾总淋巴细胞表达图谱及NCC表面的4个凝聚素受体，将开展4个凝聚素受体的类型（激活型或抑制型）鉴定与定位、分子特征的分析、凝聚素受体相互作用蛋白的筛选与功能验证以及凝聚素受体介导NCC活性调控机制的研究，从而阐明NCC表面凝聚素受体对NCC功能影响的重要性。研究结果为揭示鱼类NCC免疫监视功能的分子机理提供重要的科学理论依据，对鱼类NCC表面受体分子的挖掘与功能研究具有重要的科学意义。

Functions of nonspecific cytotoxic cell (NCC) in fish are similar to those of natural killer cell (NK cell) in higher vertebrate. It is well known for the type, function and regulation of surface receptors on NK cell, but those of NCC surface receptors are less known, which restrict to overall know the NCC function and regulation on NCC activation. Therefore this project team has obtained tilapia NCC expression map and four surface lectin receptors on NCC based on single cell sequencing technique for NCC, and then will identify the type of the four lectin receptors which belong to activating receptor or inhibitory receptor, locate the four lectin receptors in or on NCC, and analyze molecular characteristics of the four receptors, select the interacting proteins with the four receptors and verify the functions of the interacting proteins, and analyze the regulation mechanism of NCC activation mediated by the lectin receptors and elucidate the significance of the lecin receptors on the NCC function. The results of this project will provide important scientific theoretic basis for revealing molecular mechanism on immune surveillance of NCC, and have important scientific significance on the rediscovery of the surface receptors on NCC and the studies on the receptors functions.