光影响巫山淫羊藿黄酮类化合物代谢的机理及小RNA的调控作用

巫山淫羊藿Epimedium wushanense具有重要药用价值和科研价值。在对巫山淫羊藿生理生化前期研究的基础上，本项目将在分子水平上，研究光影响巫山淫羊藿生长发育和黄酮类成分代谢的机理。本项目将结合LC-MS、高通量测序等技术和生物信息学方法，系统分析光对巫山淫羊藿叶片转录组、小RNA表达谱以及黄酮类组分和含量的影响，拟构建光影响淫羊藿苷类黄酮代谢的基因表达调控网络。本项目可以获得一些在光影响巫山淫羊藿黄酮类成分代谢过程中起关键作用的功能基因和小RNA，有助于理解在不同光照条件下，小RNA与功能基因共同调控淫羊藿苷等黄酮类成分代谢的分子机理。本项目是首次系统研究小RNA在光影响淫羊藿属植物黄酮类成分代谢中的作用，研究材料、研究方法和实验设计都独具特色，有助于深入解析光影响黄酮类成分代谢的分子机理及小RNA在其中的作用，也能够为淫羊藿栽培育种及通过生物技术方法生产淫羊藿类黄酮奠定基础。

Epimedium wushanense has great value for use on medicial and scientific research. Based on the early results of physiological and biochemical studies, this research will focus on the molecular mechanism of the growth, development and flavonoid metabolism of Epimedium wushanense that affected by different light conditions. This study will apply techniques such as LC-MS, high-throughput sequencing, and some bioinformatic methods, systematically analyze the effects of light on the transcriptomes, the small RNA expression profiles, and the amount and types of flavonoids in the Epimedium wushanense leaves, and will construct the gene expression and regulation network that reflecting the effects of light on the metabolism of flavonoids such as icariin. Some functional genes or small RNAs that play significant roles in light induced biosynthesis of flavonoids such as icariin in Epimedium wushanense can be obtained in this study, and this work will be of help for understanding the molecular mechanism on small RNAs and functional genes cooperatively regulating the metabolism of flavonoids such as icariin in different light conditions. This study will systematically investigate the functions of small RNAs and the effect of light on flavonoid metabolism of Epimedium for the first time, the materials, methods and experiment designs all have paticular characteristics. This study can be help on the further analysis of the molecular mechanism of light affected flavonoid metabolism, also can be used as a basis for the cultivation and breeding of Epimedium, or the production of active ingredients of Epimedium by biotechniques.