LrMYB1调控黑果枸杞花色苷生物合成的分子机制研究

花色苷是黑果枸杞中的活性物质之一，是衡量黑果枸杞果实品质的重要指标。LrMYB1既可调节黑果枸杞植株中花色苷的合成，又参与果实的色泽形成，因此，我们推测LrMYB1在黑果枸杞花色苷的生物合成中发挥核心作用，但是，有关LrMYB1调控花色苷合成精准的分子网络还有待深入研究。本项目通过HPLC-MS/MS、转录组测序，研究野生黑果枸杞与过量表达LrMYB1的黑果枸杞5个成熟期果实中黄酮的种类、含量变化，探讨LrMYB1调控黄酮类物质代谢通路变化并促进花色苷合成的方式；结合WGCNA分析、酵母文库筛选、双萤光素酶报告基因检测及烟草瞬时表达实验，探究LrMYB1的互作蛋白及LrMYB1对下游靶基因的调控作用，最终阐明以LrMYB1为核心的黑果枸杞花色苷生物合成的分子调控网络，为后期开发分子标记，高效选育富含花色苷的黑果枸杞品种，推动黑果枸杞花色苷在医疗保健和食品工业的应用奠定基础。

Anthocyanin is one of the bioactive substances in Lycium ruthenicum Murr.(L.ruthenicum), which is an important index to measure the fruit quality of L.ruthenicum. In the previous study, we found and prove LrMYB1 can regulate the anthocyanin biosynthesis induced by salt stress in L.ruthenicum. Combined with the results of other teams on the color of the L.ruthenicum fruits, we speculated that LrMYB1 play a key role in anthocyanin biosynthesis of L.ruthenicum. However, the molecular network of LrMYB1 regulated anthocyanin biosynthesis needs to be further studied. In this project, through HPLC-MS/MS and transcriptome sequencing, we analyze the variety and content changes of flavonoids in wild L.ruthenicum and LrMYB1 overexpression fruits at five mature stages, and explored the process of the change of flavonoids in metabolic flow and anthocyanin biosynthesis regulated by LrMYB1; combined with WGCNA analysis, yeast library screening, double luciferase reporter gene detection and tobacco screening to explore the proteins interact with LrMYB1 and to analyze how LrMYB1 or the protein complex regulate downstream target genes. Results will clarify the molecular network of anthocyanin biosynthesis in L.ruthenicum regulated by LrMYB1, and lay a foundation for the development of molecular markers for breeding of L.ruthenicum varieties rich in anthocyanin to promote the application of anthocyanin in medical care and food industry.