Microbial communities of the Laurentian Great Lakes reflect connectivity and local biogeochemistry

Microbial communities of the Laurentian Great Lakes reflect connectivity and local biogeochemistry
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劳伦森五大湖的微生物群落反映了连通性和当地的生物地球化学

DOI:
10.1111/1462-2920.14862
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发表时间:
2019
影响因子:
5.1
通讯作者:
Coleman, Maureen L.
Coleman, Maureen L.
中科院分区:
生物学2区
文献类型:
--
作者:
Paver, Sara F.;Newton, Ryan J.;Coleman, Maureen L.

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劳伦森五大湖是一个巨大的,相互关联的淡水系统跨越强大的物理化学梯度,从而构成了一个强大的天然实验室,解决有关微生物生态学和进化的基本问题。我们提出了一个比较分析的浮游微生物群落在所有五个劳伦特五大湖,专注于细菌和微微型浮游生物特征通过16 S rRNA扩增子测序。我们在春季和夏季从每个湖泊的主要流域收集了2年多的样品。两种寡型,分类为LD 12(α变形菌)和acI-B1(放线菌),是每个样品中最丰富的。同时,微生物群落在夏季分层过程中表现出不同的深度模式。深层静水样本通常以Chloroflexioligotype为主,相对丰度高达19%。分层的表面社区之间的差异较冷,生产力较低的上游湖泊(上级,密歇根州,休伦)和温暖,更富有成效的下游湖泊(伊利,安大略),部分原因是由于放线菌寡型(acI-C2),平均7.7%的序列在较低的湖泊,但<0.2%的上游湖泊。总之,我们的研究结果表明,水文连通性和当地的选择性压力塑造微生物群落在五大湖,并建立一个框架,为今后的调查。
The Laurentian Great Lakes are a vast, interconnected freshwater system spanning strong physicochemical gradients, thus constituting a powerful natural laboratory for addressing fundamental questions about microbial ecology and evolution. We present a comparative analysis of pelagic microbial communities across all five Laurentian Great Lakes, focusing on Bacterial and Archaeal picoplankton characterized via 16S rRNA amplicon sequencing. We collected samples throughout the water column from the major basins of each lake in spring and summer over 2 years. Two oligotypes, classified as LD12 (Alphaproteobacteria) and acI‐B1 (Actinobacteria), were among the most abundant in every sample. At the same time, microbial communities showed distinct patterns with depth during summer stratification. Deep hypolimnion samples were frequently dominated by aChloroflexioligotype that reached up to 19% relative abundance. Stratified surface communities differed between the colder, less productive upper lakes (Superior, Michigan, Huron) and warmer, more productive lower lakes (Erie, Ontario), in part due to anActinobacteriaoligotype (acI‐C2) that averaged 7.7% of sequences in the lower lakes but <0.2% in the upper lakes. Together, our findings suggest that both hydrologic connectivity and local selective pressures shape microbial communities in the Great Lakes and establish a framework for future investigations.
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