Aminolipids elicit functional trade-offs between competitiveness and bacteriophage attachment in Ruegeria pomeroyi.
Aminolipids elicit functional trade-offs between competitiveness and bacteriophage attachment in Ruegeria pomeroyi.
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DOI：
10.1038/s41396-022-01346-0
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发表时间：
2023-03
期刊：
影响因子：
11
通讯作者：
Chen, Yin
中科院分区：
文献类型：
--
作者：
Stirrup, Rachel;Mausz, Michaela A.;Silvano, Eleonora;Murphy, Andrew;Guillonneau, Richard;Quareshy, Mussa;Rihtman, Branko;Ferretjans, Maria Aguilo;He, Ruo;Todd, Jonathan D.;Chen, Feng;Scanlan, David J.;Chen, Yin
脂质在维持细胞完整性和与周围环境的稳态方面起着至关重要的作用。世界性海洋蔷薇枝（MRC）和SAR 11分支细菌的独特之处在于，除了甘油磷脂外，它们还产生一系列含氨基酸的脂质，这些脂质通过酰胺键与β-羟基脂肪酸缀合。这些氨基脂质中的两种，鸟氨酸氨基脂质（OL）和谷氨酰胺氨基脂质（QL），使用O-乙酰转移酶OlsA合成。在这里，我们表明，OL和QL是存在于革兰氏阴性MRC细菌Ruegeria pomeroyi DSS-3的内膜和外膜。在olsA突变体中，这些氨基脂质的损失通过甘油磷脂的同时增加来补偿。无法产生氨基脂质导致膜蛋白质组发生显著变化，膜的渗透性降低，关键营养转运蛋白下调，而参与膜应激反应的蛋白质上调。事实上，进口的14 C标记的胆碱和二甲基磺基丙酸酯，作为一个代理的关键海洋营养物质跨膜运输，在olsA突变体显着受损。此外，olsA突变体的竞争力显著低于野生型（WT），在共培养中不能与WT菌株竞争。然而，不能合成这些氨基脂的olsA突变体对噬菌体附着不太敏感。总之，这些数据揭示了氨基脂在海洋细菌的这一重要分支的生态生理学中的关键作用，以及生长和避免噬菌体附着之间的权衡。
Lipids play a crucial role in maintaining cell integrity and homeostasis with the surrounding environment. Cosmopolitan marine roseobacter clade (MRC) and SAR11 clade bacteria are unique in that, in addition to glycerophospholipids, they also produce an array of amino acid-containing lipids that are conjugated with beta-hydroxy fatty acids through an amide bond. Two of these aminolipids, the ornithine aminolipid (OL) and the glutamine aminolipid (QL), are synthesized using the O-acetyltransferase OlsA. Here, we demonstrate that OL and QL are present in both the inner and outer membranes of the Gram-negative MRC bacterium Ruegeria pomeroyi DSS-3. In an olsA mutant, loss of these aminolipids is compensated by a concurrent increase in glycerophospholipids. The inability to produce aminolipids caused significant changes in the membrane proteome, with the membrane being less permeable and key nutrient transporters being downregulated while proteins involved in the membrane stress response were upregulated. Indeed, the import of 14C-labelled choline and dimethylsulfoniopropionate, as a proxy for the transport of key marine nutrients across membranes, was significantly impaired in the olsA mutant. Moreover, the olsA mutant was significantly less competitive than the wild type (WT) being unable to compete with the WT strain in co-culture. However, the olsA mutant unable to synthesize these aminolipids is less susceptible to phage attachment. Together, these data reveal a critical role for aminolipids in the ecophysiology of this important clade of marine bacteria and a trade-off between growth and avoidance of bacteriophage attachment.
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DOI：
10.1038/ismej.2015.172
发表时间：
2016-04
期刊：
The ISME journal
影响因子：
--
作者：
Sebastián M;Smith AF;González JM;Fredricks HF;Van Mooy B;Koblížek M;Brandsma J;Koster G;Mestre M;Mostajir B;Pitta P;Postle AD;Sánchez P;Gasol JM;Scanlan DJ;Chen Y
通讯作者：
Chen Y
影响因子：
5.1
作者：
Lidbury I;Kimberley G;Scanlan DJ;Murrell JC;Chen Y
通讯作者：
Chen Y
影响因子：
3.5
作者：
López-Lara, IM;Gao, JL;Geiger, O
通讯作者：
Geiger, O
影响因子：
3.6
作者：
Jaroslawski, Szymon;Duquesne, Katia;Scheuring, Simon
通讯作者：
Scheuring, Simon
影响因子：
4.4
作者：
Lynch, Alli;Tammireddy, Seshu R.;Clarke, David J.
通讯作者：
Clarke, David J.