Genome-wide identification, characterization and expression analysis of HAK genes and decoding their role in responding to potassium deficiency and abiotic stress in Medicago truncatula.
Genome-wide identification, characterization and expression analysis of HAK genes and decoding their role in responding to potassium deficiency and abiotic stress in Medicago truncatula.
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作者：
Zhao Y;Wang L;Zhao P;Liu Z;Guo S;Li Y;Liu H
HAK家族是最大的钾（K+）转运蛋白家族，在K+吸收、植物生长以及植物生物和非生物胁迫响应中至关重要。尽管HAK家族成员在许多物种中已被鉴定并进行了功能研究，但这些基因在蒺藜苜蓿（Medicago truncatula）中仍然没有详细研究，蒺藜苜蓿是研究豆科植物遗传学的良好模式系统。在这项研究中，我们筛选了M。蒺藜HAK家族成员（MtHAKs）。此外，我们还进行了鉴定，系统发育分析，并预测MtHAKs的保守模体。此外，我们研究了在K+缺乏，干旱和盐胁迫下的MtHAKs的表达水平，使用定量实时PCR（qRT-PCR）。我们确定了20个MtHAK家族成员，并根据系统发育关系将其分为三个簇。保守基序分析表明，除MtHAK 10外，所有MtHAK蛋白均含有高度保守的K+转运结构域（GVVYGDLGTSAK）。qRT-PCR分析表明，根中的多个MtHAK基因受到非生物胁迫的诱导。特别是，MtHAK 15、MtHAK 17和MtHAK 18在M.在缺钾、干旱和盐胁迫条件下，这些基因对蒺藜根的生长有显著影响，从而表明这些基因是高亲和力K+吸收的良好候选者，因此在耐旱和耐盐性中具有重要作用。我们的研究结果不仅首次对M.同时也揭示了豆科作物对缺钾和非生物胁迫响应的潜在信息，为今后豆科作物抗逆分子育种奠定了基础。
The HAK family is the largest potassium (K+) transporter family, vital in K+ uptake, plant growth, and both plant biotic and abiotic stress responses. Although HAK family members have been characterized and functionally investigated in many species, these genes are still not studied in detail in Medicago truncatula, a good model system for studying legume genetics. In this study, we screened the M. truncatula HAK family members (MtHAKs). Furthermore, we also conducted the identification, phylogenetic analysis, and prediction of conserved motifs of MtHAKs. Moreover, we studied the expression levels of MtHAKs under K+ deficiency, drought, and salt stresses using quantitative real-time PCR (qRT-PCR). We identified 20 MtHAK family members and classified them into three clusters based on phylogenetic relationships. Conserved motif analyses showed that all MtHAK proteins besides MtHAK10 contained the highly conserved K+ transport domain (GVVYGDLGTSPLY). qRT-PCR analysis showed that several MtHAK genes in roots were induced by abiotic stress. In particular, MtHAK15, MtHAK17, and MtHAK18 were strongly up-regulated in the M. truncatula roots under K+ deficiency, drought, and salt stress conditions, thereby implying that these genes are good candidates for high-affinity K+ uptake and therefore have essential roles in drought and salt tolerance. Our results not only provided the first genetic description and evolutionary relationships of the K+ transporter family in M. truncatula, but also the potential information responding to K+ deficiency and abiotic stresses, thereby laying the foundation for molecular breeding of stress-resistant legume crops in the future.
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