Type V collagen regulates the structure and biomechanics of TMJ condylar cartilage: A fibrous-hyaline hybrid.
Type V collagen regulates the structure and biomechanics of TMJ condylar cartilage: A fibrous-hyaline hybrid.
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DOI：
10.1016/j.matbio.2021.07.002
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Han L
中科院分区：
文献类型：
--
作者：
Chandrasekaran P;Kwok B;Han B;Adams SM;Wang C;Chery DR;Mauck RL;Dyment NA;Lu XL;Frank DB;Koyama E;Birk DE;Han L
这项研究质疑V型胶原在颞下颌关节(TMJ)髁状软骨出生后生长中的作用，TMJ是一种混合组织，具有纤维软骨层覆盖次级透明软骨层。结合组织学、免疫荧光成像、电子显微镜和原子力显微镜纳米力学测试的结果，我们阐明了V型胶原减少对V型胶原单倍化和诱导基因敲除小鼠TMJ髁突软骨生长的影响。V型胶原减少导致胶原纤维显著增厚，组织弹性系数降低，细胞密度降低，纤维层和透明层细胞聚集异常。出生后的髁突软骨生长包括位于纤维层的前体细胞的软骨生成，这导致了次级透明层的形成。V型胶原的丢失导致这些细胞的增殖减少，提示V型胶原可能在调节祖细胞生态位中发挥作用。在开始生理性TMJ负荷后，当在断奶后的小鼠中诱导V型胶原被敲除时，透明层明显变薄，支持了V型胶原与咬合负荷之间的相互作用，从而促进了髁突软骨的生长。因此，透明层的表型可以归因于V型胶原对机械调节的祖细胞活动的影响。相反，膝关节软骨在出生后阶段不包含祖细胞群体，并发展出正常的结构和生物力学性能，但V型胶原丢失。因此，在TMJ中，V型胶原除了调节I型胶原纤维的组装外，还影响纤维层祖细胞活动的机械调节。我们期望这些知识为了解髁状突软骨基质的发育和再生奠定基础，并对典型Ehler-Danlos综合征患者的TMJ症状产生新的见解，这是一种由V型胶原常染色体突变引起的遗传病。
This study queried the role of type V collagen in the post-natal growth of temporomandibular joint (TMJ) condylar cartilage, a hybrid tissue with a fibrocartilage layer covering a secondary hyaline cartilage layer. Integrating outcomes from histology, immunofluorescence imaging, electron microscopy and atomic force microscopy-based nanomechanical tests, we elucidated the impact of type V collagen reduction on TMJ condylar cartilage growth in the type V collagen haploinsufficiency and inducibleknockout mice. Reduction of type V collagen led to significantly thickened collagen fibrils, decreased tissue modulus, reduced cell density and aberrant cell clustering in both the fibrous and hyaline layers. Post-natal growth of condylar cartilage involves the chondrogenesis of progenitor cells residing in the fibrous layer, which gives rise to the secondary hyaline layer. Loss of type V collagen resulted in reduced proliferation of these cells, suggesting a possible role of type V collagen in mediating the progenitor cell niche. When the knockout of type V collagen was induced in post-weaning mice after the start of physiologic TMJ loading, the hyaline layer exhibited pronounced thinning, supporting an interplay between type V collagen and occlusal loading in condylar cartilage growth. The phenotype in hyaline layer can thus be attributed to the impact of type V collagen on the mechanically regulated progenitor cell activities. In contrast, knee cartilage does not contain the progenitor cell population at post-natal stages, and develops normal structure and biomechanical properties with the loss of type V collagen. Therefore, in the TMJ, in addition to its established role in regulating the assembly of collagen I fibrils, type V collagen also impacts the mechanoregulation of progenitor cell activities in the fibrous layer. We expect such knowledge to establish a foundation for understanding condylar cartilage matrix development and regeneration, and to yield new insights into the TMJ symptoms in patients with classic Ehlers-Danlos syndrome, a genetic disease due to autosomal mutation of type V collagen.
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DOI：
10.4061/2011/212385
发表时间：
2011-01-01
期刊：
Journal of dental biomechanics
影响因子：
--
作者：
Hagandora, Catherine K;Chase, Thomas W;Almarza, Alejandro J
通讯作者：
Almarza, Alejandro J
影响因子：
6.5
作者：
Bonod-Bidaud, Christelle;Roulet, Muriel;Ruggiero, Florence
通讯作者：
Ruggiero, Florence
影响因子：
3.7
作者：
Dutra EH;O' Brien MH;Lima A;Kalajzic Z;Tadinada A;Nanda R;Yadav S
通讯作者：
Yadav S
影响因子：
9.7
作者：
Chery, Daphney R.;Han, Biao;Han, Lin
通讯作者：
Han, Lin
影响因子：
4.4
作者：
Connizzo, Brianne K.;Han, Lin;Soslowsky, Louis J.
通讯作者：
Soslowsky, Louis J.