Cellular and Matrix Response of the Mandibular Condylar Cartilage to Botulinum Toxin.
Cellular and Matrix Response of the Mandibular Condylar Cartilage to Botulinum Toxin.
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DOI：
10.1371/journal.pone.0164599
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Yadav S
中科院分区：
文献类型：
--
作者：
Dutra EH;O' Brien MH;Lima A;Kalajzic Z;Tadinada A;Nanda R;Yadav S
探讨A型肉毒杆菌毒素（Botox）对下颌髁突软骨（MCC）和髁突下骨的细胞和基质作用。在第1天将肉毒杆菌毒素（0.3单位）注射到5周龄转基因小鼠（Col 10a 1-RFPcherry）的右咬肌中。左侧咬肌作为动物内对照。腹膜内注射以下骨标记物：第7天的钙黄绿素，第14天的茜素红和第21天的钙黄绿素。此外，在处死前48小时和24小时注射EdU。在肉毒杆菌注射后30天处死小鼠。解剖实验侧和对照侧下颌骨，并通过X线成像和显微CT进行检查。随后，将MCC与软骨下骨一起沿着切片，并用抗酒石酸酸性磷酸酶（TRAP）、EdU、TUNEL、碱性磷酸酶、甲苯胺蓝和番红O染色。此外，我们还对pSMAD和VEGF进行了免疫组化。与左侧相比，右侧的软骨下骨和矿化软骨（注射肉毒杆菌毒素）的骨体积分数、组织密度和骨小梁厚度显著降低。注射肉毒素后，下颌骨长度和髁突头长度无显著差异，但髁突宽度明显减小。我们的组织学显示，在软骨下骨和下颌髁状软骨中表达Col 10a 1的细胞数量减少，细胞增殖减少，细胞凋亡增加，注射肉毒杆菌毒素的侧软骨和软骨下骨的TRAP活性和矿化降低。此外，我们观察到与对照侧相比，在肉毒杆菌注射侧的MCC中蛋白聚糖和糖胺聚糖分布减少，并且pSMAD 1/5/8和VEGF表达减少。在咬肌中注射肉毒杆菌毒素导致MCC和软骨下骨中矿化和基质沉积减少，软骨细胞增殖和分化减少，细胞凋亡增加。
To evaluate the cellular and matrix effects of botulinum toxin type A (Botox) on mandibular condylar cartilage (MCC) and subchondral bone. Botox (0.3 unit) was injected into the right masseter of 5-week-old transgenic mice (Col10a1-RFPcherry) at day 1. Left side masseter was used as intra-animal control. The following bone labels were intraperitoneally injected: calcein at day 7, alizarin red at day 14 and calcein at day 21. In addition, EdU was injected 48 and 24 hours before sacrifice. Mice were sacrificed 30 days after Botox injection. Experimental and control side mandibles were dissected and examined by x-ray imaging and micro-CT. Subsequently, MCC along with the subchondral bone was sectioned and stained with tartrate resistant acid phosphatase (TRAP), EdU, TUNEL, alkaline phosphatase, toluidine blue and safranin O. In addition, we performed immunohistochemistry for pSMAD and VEGF. Bone volume fraction, tissue density and trabecular thickness were significantly decreased on the right side of the subchondral bone and mineralized cartilage (Botox was injected) when compared to the left side. There was no significant difference in the mandibular length and condylar head length; however, the condylar width was significantly decreased after Botox injection. Our histology showed decreased numbers of Col10a1 expressing cells, decreased cell proliferation and increased cell apoptosis in the subchondral bone and mandibular condylar cartilage, decreased TRAP activity and mineralization of Botox injected side cartilage and subchondral bone. Furthermore, we observed reduced proteoglycan and glycosaminoglycan distribution and decreased expression of pSMAD 1/5/8 and VEGF in the MCC of the Botox injected side in comparison to control side. Injection of Botox in masseter muscle leads to decreased mineralization and matrix deposition, reduced chondrocyte proliferation and differentiation and increased cell apoptosis in the MCC and subchondral bone.
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DOI：
10.1242/dev.029926
发表时间：
2009-04
期刊：
Development (Cambridge, England)
影响因子：
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作者：
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影响因子：
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DOI：
10.1179/crn.2008.017
发表时间：
2008-04-01
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DOI：
10.1073/pnas.96.6.3200
发表时间：
1999-03-16
影响因子：
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作者：
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