A rat model of central venous catheter to study establishment of long-term bacterial biofilm and related acute and chronic infections.
A rat model of central venous catheter to study establishment of long-term bacterial biofilm and related acute and chronic infections.
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DOI：
10.1371/journal.pone.0037281
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Beloin C
中科院分区：
文献类型：
--
作者：
Chauhan A;Lebeaux D;Decante B;Kriegel I;Escande MC;Ghigo JM;Beloin C
在病原微生物定殖的医疗器械上形成弹性生物膜是医疗保健相关感染的主要原因。虽然体外生物膜分析带来了有前景的抗生物膜方法，但人们对它们在体内情况的转化以及宿主对医疗器械感染体内动态的贡献知之甚少。在这里，我们开发了一种长期细菌生物膜感染的体内模型，该模型通过手术放置在成年大鼠的儿科完全植入式静脉通路（TIVAP）中。利用非侵入性定量生物发光，我们研究了临床相关病原体、大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和表皮葡萄球菌对 TIVAP 的污染，并证明 TIVAP 细菌群体表现出典型的生物膜表型。在我们的研究中，我们发现免疫功能健全的大鼠能够控制定植并清除血流感染，除了高达 30% 的大鼠遭受全身感染和死亡，而免疫抑制的大鼠没有一只在感染中幸存。此外，我们还模拟了一些临床相关的 TIVAP 相关并发症，例如端口袋感染和血行定植途径。最后，通过评估优化的抗生素封闭疗法，我们确定我们的体内模型能够评估针对细菌生物膜感染的创新治疗策略。
Formation of resilient biofilms on medical devices colonized by pathogenic microorganisms is a major cause of health-care associated infection. While in vitro biofilm analyses led to promising anti-biofilm approaches, little is known about their translation to in vivo situations and on host contribution to the in vivo dynamics of infections on medical devices. Here we have developed an in vivo model of long-term bacterial biofilm infections in a pediatric totally implantable venous access port (TIVAP) surgically placed in adult rats. Using non-invasive and quantitative bioluminescence, we studied TIVAP contamination by clinically relevant pathogens, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis, and we demonstrated that TIVAP bacterial populations display typical biofilm phenotypes. In our study, we showed that immunocompetent rats were able to control the colonization and clear the bloodstream infection except for up to 30% that suffered systemic infection and death whereas none of the immunosuppressed rats survived the infection. Besides, we mimicked some clinically relevant TIVAP associated complications such as port-pocket infection and hematogenous route of colonization. Finally, by assessing an optimized antibiotic lock therapy, we established that our in vivo model enables to assess innovative therapeutic strategies against bacterial biofilm infections.
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