Genotypes and mouse virulence of Toxoplasma gondii isolates from animals and humans in China.
Genotypes and mouse virulence of Toxoplasma gondii isolates from animals and humans in China.
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DOI：
10.1371/journal.pone.0053483
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shen J
中科院分区：
文献类型：
--
作者：
Wang L;Chen H;Liu D;Huo X;Gao J;Song X;Xu X;Huang K;Liu W;Wang Y;Lu F;Lun ZR;Luo Q;Wang X;Shen J
近年来对弓形虫种群结构的研究表明，弓形虫在不同的地理区域有几个主要的无性系。南美洲的弓形虫在遗传和生物学上是不同的，而这种寄生虫在北美和欧洲是明显的无性繁殖，有几个主要的谱系，包括I型，II型和III型。关于中国弓形虫分离株的基因型和小鼠毒力的信息很少，不足以调查其种群结构、进化和传播。采用10个标记（SAG1、SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico）对23株不同寄主弓形虫分离物进行基因分型和限制性片段长度多态性分析；其中3个基因型为非典型基因型，2个为原型基因型。15个毒株属于中国1型毒株，该毒株以前曾报道在中国广泛存在于猪、猫和人身上。2株人类分离株属于I型和II型毒系，其余分离株属于国内未报道的2种新的非典型基因型（ToxoDB#204和#205）。结果表明，除TgCtwh6菌株外，其他基因型的弓形虫分离株在小鼠体内均具有中等或高度毒力，TgCtwh6菌株在感染后35天内仍在小鼠体内保持寄生虫血症，尽管它具有统一的中国1型基因型。此外，所有基因型china 1分离株的系统发育网络分析均相同，4个内含子（EF1、HP2、UPRT1和UPRT7）和2个致密颗粒蛋白（GRA6和GRA7）的序列数据均无差异。发现遗传多样性有限，中国大陆主要流行中国1基因型（弓形虫b #9）。研究结果将为深入了解中国弓形虫种群结构、流行病学和生物学特性提供有益的资料。
Recent population structure studies of T. gondii revealed that a few major clonal lineages predominated in different geographical regions. T. gondii in South America is genetically and biologically divergent, whereas this parasite is remarkably clonal in North America and Europe with a few major lineages including Types I, II and III. Information on genotypes and mouse virulence of T. gondii isolates from China is scarce and insufficient to investigate its population structure, evolution, and transmission. Genotyping of 23 T. gondii isolates from different hosts using 10 markers for PCR-restriction fragment length polymorphism analyses (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico) revealed five genotypes; among them three genotypes were atypical and two were archetypal. Fifteen strains belong to the Chinese 1 lineage, which has been previously reported as a widespread lineage from swine, cats, and humans in China. Two human isolates fall into the type I and II lineages and the remaining isolates belong to two new atypical genotypes (ToxoDB#204 and #205) which has never been reported in China. Our results show that these genotypes of T. gondii isolates are intermediately or highly virulent in mice except for the strain TgCtwh6, which maintained parasitemia in mice for 35 days post infection although it possesses the uniform genotype of Chinese 1. Additionally, phylogenetic network analyses of all isolates of genotype Chinese 1 are identical, and there is no variation based on the sequence data generated for four introns (EF1, HP2, UPRT1 and UPRT7) and two dense granule proteins (GRA6 and GRA7). A limited genetic diversity was found and genotype Chinese 1 (ToxoDB#9) is dominantly circulating in mainland China. The results will provide a useful profile for deep insight to the population structure, epidemiology and biological characteristics of T. gondii in China.
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影响因子：
3.7
作者：
Jamieson, Sarra E.;de Roubaix, Lee-Anne;Cortina-Borja, Mario;Tan, Hooi Kuan;Mui, Ernest J.;Cordell, Heather J.;Kirisits, Michael J.;Miller, E. Nancy;Peacock, Christopher S.;Hargrave, Aubrey C.;Coyne, Jessica J.;Boyer, Kenneth;Bessieres, Marie-Helene;Buffolano, Wilma;Ferret, Nicole;Franck, Jacqueline;Kieffer, Francois;Meier, Paul;Nowakowska, Dorota E.;Paul, Malgorzata;Peyron, Francois;Stray-Pedersen, Babill;Prusa, Andrea-Romana;Thulliez, Philippe;Wallon, Martine;Petersen, Eskild;McLeod, Rima;Gilbert, Ruth E.;Blackwell, Jenefer M.
通讯作者：
Blackwell, Jenefer M.
影响因子：
4
作者：
Dubey, J. P.;Velmurugan, G. V.;Su, C.
通讯作者：
Su, C.
影响因子：
2.6
作者：
Dubey, JP
通讯作者：
Dubey, JP
影响因子：
3.1
作者：
Howe, DK;Summers, BC;Sibley, LD
通讯作者：
Sibley, LD
影响因子：
6.6
作者：
Carruthers, Vern B.;Suzuki, Yasuhiro
通讯作者：
Suzuki, Yasuhiro