Dual function of OmpM as outer membrane tether and nutrient uptake channel in diderm Firmicutes.
Dual function of OmpM as outer membrane tether and nutrient uptake channel in diderm Firmicutes.
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DOI：
10.1038/s41467-023-42601-y
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发表时间：
2023-11-06
影响因子：
16.6
通讯作者：
van den Berg, Bert
中科院分区：
文献类型：
--
作者：
Silale, Augustinas;Zhu, Yiling;Witwinowski, Jerzy;Smith, Robert E.;Newman, Kahlan E.;Bhamidimarri, Satya P.;Basle, Arnaud;Khalid, Syma;Beloin, Christophe;Gribaldo, Simonetta;van den Berg, Bert
为了机械稳定性和维持细胞形态，革兰氏阴性细菌的外膜（OM）必须被拴在肽聚糖上。来自terrabobacteria组的大多数diderm门最近被证明缺乏明确的OM附着系统，但取而代之的是OmpM，这可能代表了细菌中祖先的拴系系统。在这里，我们用单粒子低温电子显微镜确定了最丰富的OmpM蛋白的结构，这些蛋白来自小叶细孔菌（厚壁菌门）。我们还表征了OmpM的跨膜β-桶的通道特性，并研究了其质周柄区域的结构和pg结合特性。我们的研究结果表明，细小弧菌和其他diderm terrabobacteria的OM拴住和营养获取在遗传上是相关的。OmpM的这种双重功能可能在祖先细菌中OM的丧失和单胚层细菌谱系的出现中发挥了作用。外膜系结对双层细菌的包膜完整性具有重要意义。本文对terrabobacteria主要的外膜系索系统——有柄孔蛋白OmpM进行了结构和功能分析。
The outer membrane (OM) in diderm, or Gram-negative, bacteria must be tethered to peptidoglycan for mechanical stability and to maintain cell morphology. Most diderm phyla from the Terrabacteria group have recently been shown to lack well-characterised OM attachment systems, but instead have OmpM, which could represent an ancestral tethering system in bacteria. Here, we have determined the structure of the most abundant OmpM protein from Veillonella parvula (diderm Firmicutes) by single particle cryogenic electron microscopy. We also characterised the channel properties of the transmembrane β-barrel of OmpM and investigated the structure and PG-binding properties of its periplasmic stalk region. Our results show that OM tethering and nutrient acquisition are genetically linked in V. parvula, and probably other diderm Terrabacteria. This dual function of OmpM may have played a role in the loss of the OM in ancestral bacteria and the emergence of monoderm bacterial lineages. Outer membrane tethering is important for cell envelope integrity in diderm bacteria. Here, the authors present structures and functional analyses of the stalked porin OmpM, which is the main outer membrane tethering system within the Terrabacteria.
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DOI：
10.1107/s0907444905036693
发表时间：
2006-01-01
影响因子：
2.2
作者：
Evans, P
通讯作者：
Evans, P
DOI：
10.1016/j.jbc.2022.101745
发表时间：
2022-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Legg MSG;Hager-Mair FF;Krauter S;Gagnon SML;Lòpez-Guzmán A;Lim C;Blaukopf M;Kosma P;Schäffer C;Evans SV
通讯作者：
Evans SV
影响因子：
3.2
作者：
BLADEN, HA;MERGENHAGEN, SE
通讯作者：
MERGENHAGEN, SE
影响因子：
5.6
作者：
Basle, Arnaud;Rummel, Gabriele;Schirmer, Tilman
通讯作者：
Schirmer, Tilman
影响因子：
17.1
作者：
Bajaj, Harsha;Gutierrez, Silvia Acosta;Ceccarelli, Matteo
通讯作者：
Ceccarelli, Matteo