Identification, Characterization, and Stress Responsiveness of Glucose-6-phosphate Dehydrogenase Genes in Highland Barley
Identification, Characterization, and Stress Responsiveness of Glucose-6-phosphate Dehydrogenase Genes in Highland Barley
复制标题
青稞中 6-磷酸葡萄糖脱氢酶基因的鉴定、表征和胁迫响应
DOI：
10.3390/plants9121800
复制
发表时间：
2020-12-18
期刊：
影响因子：
--
通讯作者：
Bi Y
中科院分区：
文献类型：
--
作者：
Feng R;Wang X;He L;Wang S;Li J;Jin J;Bi Y
G6 PDH为植物代谢提供中间代谢产物和还原力（烟酰胺腺嘌呤二核苷酸磷酸，NADPH），在细胞氧化还原稳态中发挥关键作用。本研究克隆了五个青稞G6 PDH基因（HvG 6 HH 1至HvG 6 HH 5），并对其编码的蛋白质进行了表征。HvG 6PDHs在大肠杆菌中的功能分析大肠杆菌中表达的G6 PDH蛋白表明，HvG 6PDH 1至HvG 6PDH 5编码功能性G6 PDH蛋白。亚细胞定位和系统发育分析表明，HvG 6PDH 2和HvG 6PDH 5定位于细胞质中，而HvG 6PDH 1，HvG 6PDH 3和HvG 6PDH 4是质体异构体。酶活性和基因表达分析表明，HvG 6PDH 1至HvG 6PDH 4参与响应盐和干旱胁迫。胞质HvG 6PDH 2是抗氧化应激的主要同种型。HvG 6PDH 5可能是一个管家基因。此外，茉莉酸（JA）和脱落酸（阿坝）处理后，HvG 6PDH 1 ~ HvG 6PDH 4及其编码的酶均产生了响应，表明JA和阿坝可能是HvG 6PDH 5的关键调节因子。活性氧分析表明，在盐和干旱胁迫下，细胞质和质体G6 PDH活性的抑制导致了青稞中H2 O2和O2−含量的增加。这些结果表明，G6 PDH可以维持细胞的氧化还原稳态，胞质HvG 6PDH 2是一个不可替代的异构体，在青稞抗氧化应激。
G6PDH provides intermediate metabolites and reducing power (nicotinamide adenine dinucleotide phosphate, NADPH) for plant metabolism, and plays a pivotal role in the cellular redox homeostasis. In this study, we cloned five G6PDH genes (HvG6PDH1 to HvG6PDH5) from highland barley and characterized their encoded proteins. Functional analysis of HvG6PDHs in E. coli showed that HvG6PDH1 to HvG6PDH5 encode the functional G6PDH proteins. Subcellular localization and phylogenetic analysis indicated that HvG6PDH2 and HvG6PDH5 are localized in the cytoplasm, while HvG6PDH1, HvG6PDH3, and HvG6PDH4 are plastidic isoforms. Analysis of enzymatic activities and gene expression showed that HvG6PDH1 to HvG6PDH4 are involved in responses to salt and drought stresses. The cytosolic HvG6PDH2 is the major isoform against oxidative stress. HvG6PDH5 may be a house-keeping gene. In addition, HvG6PDH1 to HvG6PDH4 and their encoded enzymes responded to jasmonic acid (JA) and abscisic acid (ABA) treatments, implying that JA and ABA are probably critical regulators of HvG6PDHs (except for HvG6PDH5). Reactive oxygen species analysis showed that inhibition of cytosolic and plastidic G6PDH activities leads to increased H2O2 and O2− contents in highland barley under salt and drought stresses. These results suggest that G6PDH can maintain cellular redox homeostasis and that cytosolic HvG6PDH2 is an irreplaceable isoform against oxidative stress in highland barley.
登录
查看更多内容
影响因子：
5.7
作者：
Foyer CH
通讯作者：
Foyer CH
DOI：
10.1073/pnas.1215265109
发表时间：
2012-10-16
影响因子：
11.1
作者：
Dai, Fei;Nevo, Eviatar;Zhang, Guoping
通讯作者：
Zhang, Guoping
影响因子：
4.5
作者：
He, Li;Wang, Xiaomin;Bi, Yurong
通讯作者：
Bi, Yurong
影响因子：
5.2
作者：
Honjoh, Kenichi;Machida, Takeshi;Iio, Masayoshi
通讯作者：
Iio, Masayoshi
影响因子：
1.6
作者：
Acero-Navarro, Kevin E.;Jimenez-Ramirez, Mariella;Velasco-Garcia, Roberto
通讯作者：
Velasco-Garcia, Roberto